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Role of DNA repair in Bacillus subtilis spore resistance to high energy and low energy electron beam treatments.
Zhang, Yifan; Huber, Nina; Moeller, Ralf; Stülke, Jörg; Dubovcova, Barbora; Akepsimaidis, Georgios; Meneses, Nicolas; Drissner, David; Mathys, Alexander.
Afiliación
  • Zhang Y; Sustainable Food Processing Laboratory, Institute of Food, Nutrition and Health, Department of Health Science and Technology, ETH Zurich, Zurich, Switzerland.
  • Huber N; Sustainable Food Processing Laboratory, Institute of Food, Nutrition and Health, Department of Health Science and Technology, ETH Zurich, Zurich, Switzerland.
  • Moeller R; Space Microbiology Research Group, Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center, Cologne (Köln), Germany.
  • Stülke J; Department of General Microbiology, Georg-August-University Göttingen, Göttingen, Germany.
  • Dubovcova B; Data Analytics & Services, Digital Technologies Business Area, Bühler AG, Uzwil, Switzerland.
  • Akepsimaidis G; Data Analytics & Services, Digital Technologies Business Area, Bühler AG, Uzwil, Switzerland.
  • Meneses N; Data Analytics & Services, Digital Technologies Business Area, Bühler AG, Uzwil, Switzerland.
  • Drissner D; Microbiology, Food Hygiene and Consumer Health Protection, Department of Life Sciences, Albstadt- Sigmaringen University, Sigmaringen, Germany.
  • Mathys A; Sustainable Food Processing Laboratory, Institute of Food, Nutrition and Health, Department of Health Science and Technology, ETH Zurich, Zurich, Switzerland. Electronic address: alexander.mathys@hest.ethz.ch.
Food Microbiol ; 87: 103353, 2020 May.
Article en En | MEDLINE | ID: mdl-31948638
Bacillus subtilis spore inactivation mechanisms under low energy electron beam (LEEB) and high energy electron beam (HEEB) treatment were investigated using seven mutants lacking specific DNA repair mechanisms. The results showed that most of the DNA repair-deficient mutants, including ΔrecA, ΔKu ΔligD, Δexo Δnfo, ΔuvrAB and ΔsbcDC, had reduced resistances towards electron beam (EB) treatments at all investigated energy levels (80 keV, 200 keV and 10 MeV) compared to their wild type. This result suggested DNA damage was induced during EB treatments. The mutant lacking recA showed the lowest resistance, followed by the mutant lacking Ku and ligD. These findings indicated that recA, Ku and ligD and their associated DNA repair mechanisms, namely, homologous recombination and non-homologous end joining, play important roles in spore survival under EB treatment. Furthermore, exoA, nfo, uvrAB, splB, polY1 and polY2, which are involved in nucleotide damage repair/removal, showed different levels of effects on spore resistance under EB treatment. Finally, the results suggested that HEEB and LEEB inactivate B. subtilis spores through similar mechanisms. This research will provide a better understanding of how EB technologies inactivate B. subtilis spores and will contribute to the application of these technologies as a non-thermal, gentle spore control approach.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas Bacterianas / Bacillus subtilis / Reparación del ADN Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Esporas Bacterianas / Bacillus subtilis / Reparación del ADN Idioma: En Revista: Food Microbiol Asunto de la revista: CIENCIAS DA NUTRICAO / MICROBIOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Suiza Pais de publicación: Reino Unido