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Rapid isolation of mouse ILCs from murine intestinal tissues.
Burrows, Kyle; Chiaranunt, Pailin; Ngai, Louis; Mortha, Arthur.
Afiliación
  • Burrows K; Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • Chiaranunt P; Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • Ngai L; Department of Immunology, University of Toronto, Toronto, ON, Canada.
  • Mortha A; Department of Immunology, University of Toronto, Toronto, ON, Canada. Electronic address: arthur.mortha@utoronto.ca.
Methods Enzymol ; 631: 305-327, 2020.
Article en En | MEDLINE | ID: mdl-31948554
Tissue-resident immune cells like innate lymphoid cells (ILCs) are regulators of local immunity and tissue homeostasis. Similar to Natural Killer (NK) cells, ILCs express germline-encoded natural cytotoxicity receptors (NCRs) that facilitate the rapid execution of effector functions. Recent advances using transgenic animal models have further uncovered the developmental, transcriptional, epigenetic, and functional differences between members of the ILC family. Isolation of ILCs, which are particularly enriched in non-lymphoid tissues, can often be challenging and time consuming. Here, we provide a simple and rapid protocol for the isolation of NK cells and ILCs from murine intestinal tissues. This protocol is suitable for Fluorescence Activated Cell Sorting (FACS) and intracellular analysis of cytokine and transcription factor expression using flow and mass cytometry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos / Separación Celular / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Methods Enzymol Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos / Separación Celular / Intestinos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Methods Enzymol Año: 2020 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos