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Continuous flow reactor based with an immobilized biocatalyst for the continuous enzymatic transesterification of crude coconut oil.
Santana, Juliana L; Oliveira, Juliana M; Nascimento, Jamily S; Mattedi, Silvana; Krause, Laiza C; Freitas, Lisiane S; Cavalcanti, Eliane B; Pereira, Matheus M; Lima, Álvaro S; Soares, Cleide M F.
Afiliación
  • Santana JL; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Oliveira JM; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Nascimento JS; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Mattedi S; Departamento de Engenharia Química, Universidade Federal da Bahia, Salvador, BA, Brazil.
  • Krause LC; Núcleo de Estudo em Sistemas Coloidais-NUESC, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Freitas LS; Departamento de Química, Universidade Federal de Sergipe, São Cristóvão, Sergipe, SE, Brazil.
  • Cavalcanti EB; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Pereira MM; CICECO, Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal.
  • Lima ÁS; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
  • Soares CMF; Instituto de Tecnologia e Pesquisa-ITP, Universidade Tiradentes, Aracaju, SE, Brazil.
Biotechnol Appl Biochem ; 67(3): 404-413, 2020 May.
Article en En | MEDLINE | ID: mdl-31930535
Here, we have assessed the use of one packed bed or two packed bed reactors in series in which Burkholderia cepacia lipase (BCL) was immobilized on protic ionic liquid (PIL)-modified silica and used as a biocatalyst for the transesterification of crude coconut oil. Reaction parameters including volumetric flow, temperature, and molar ratio were evaluated. The conversion of transesterification reaction products (ethyl esters) was determined using gas chromatography and the quantities of intermediate products (diglyceride and monoglyceride [MG]) were assessed using high-performance liquid chromatography. Packed bed reactors in series produced ethyl esters with the greatest efficiency, achieving 65.27% conversion after 96 H at a volumetric flow rate of 0.50 mL Min-1 at 40 °C and a 1:9 molar ratio of oil to ethanol. Further, within the first 24 H of the reaction, increased MG (54.5%) production was observed. Molecular docking analyses were performed to evaluate the catalytic step of coconut oil transesterification in the presence of BCL. Molecular docking analysis showed that triglycerides have a higher affinity energy (-5.7 kcal mol-1 ) than the smallest MG (-6.0 kcal mol-1 ), therefore, BCL catalyzes the conversion of triglycerides rather than MG, which is consistent with experimental results.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reactores Biológicos / Ésteres / Aceite de Coco / Lipasa Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reactores Biológicos / Ésteres / Aceite de Coco / Lipasa Idioma: En Revista: Biotechnol Appl Biochem Asunto de la revista: BIOQUIMICA / BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos