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Autophagy protects PC12 cells against deoxynivalenol toxicity via the Class III PI3K/beclin 1/Bcl-2 pathway.
Wang, Xichun; Jiang, Yunjing; Zhu, Lei; Cao, Li; Xu, Wei; Rahman, Sajid Ur; Feng, Shibin; Li, Yu; Wu, Jinjie.
Afiliación
  • Wang X; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Jiang Y; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Zhu L; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Cao L; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Xu W; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Rahman SU; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Feng S; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Li Y; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Wu J; Department of Veterinary Medicine, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
J Cell Physiol ; 235(11): 7803-7815, 2020 11.
Article en En | MEDLINE | ID: mdl-31930515
Deoxynivalenol (DON) is a major mycotoxin from the trichothecene family of mycotoxins produced by Fusarium fungi. It can cause a variety of adverse effects on human and farm animal health. Here, we determined the effect of DON on the Class III phosphatidylinositol 3-kinase (PIK3C3)/beclin 1/B cell lymphoma-2 (Bcl-2) pathway in PC12 cells and the relationship between autophagy and apoptosis. The effects of DON were evaluated based on the apoptosis ratio; the typical indicators of autophagy, including cellular morphology, acridine orange- and monodansylcadaverine-labeled vacuoles, green fluorescent protein-microtubule associated protein 1 light chain 3 (LC3) localization, and LC3 immunofluorescence; and the expression of key autophagy-related genes and proteins, that is, PIK3C3, beclin 1, Bcl-2, LC3, and p62. The relationship between autophagy and apoptosis was analyzed by western blot analysis and flow cytometry. DON-induced PC12 cell morphological changes and autophagy significantly. PIK3C3, beclin 1, and LC3 increased in tandem with the DON concentration used; Bcl-2 and p62 expression decreased as DON concentrations increased. Moreover, the PIK3C3/beclin 1/Bcl-2 signaling pathway played a role in DON-induced autophagy. Our findings suggest that DON can induce autophagy by activating the PIK3C3/beclin 1/Bcl-2 signaling pathway and that autophagy may play a positive role in reducing DON-induced apoptosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Tricotecenos / Proteínas Proto-Oncogénicas c-bcl-2 / Fosfatidilinositol 3-Quinasas / Beclina-1 Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Tricotecenos / Proteínas Proto-Oncogénicas c-bcl-2 / Fosfatidilinositol 3-Quinasas / Beclina-1 Límite: Animals Idioma: En Revista: J Cell Physiol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos