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Endothelial cells support osteogenesis in an in vitro vascularized bone model developed by 3D bioprinting.
Chiesa, Irene; De Maria, Carmelo; Lapomarda, Anna; Fortunato, Gabriele Maria; Montemurro, Francesca; Di Gesù, Roberto; Tuan, Rocky S; Vozzi, Giovanni; Gottardi, Riccardo.
Afiliación
  • Chiesa I; Research Center 'E. Piaggio', University of Pisa, Pisa, Italy. Center for Cellular and Molecular Engineering, Department of Orthopedic Surgery, School of Medicine, University of Pittsburgh, Pittsburgh, United States of America. Department of Pediatrics, Division of Pulmonary Medicine, The Children's Hospital of Philadelphia, Philadelphia, United States of America. Dept. of Ingegneria dell'Informazione, University of Pisa, Pisa, Italy.
Biofabrication ; 12(2): 025013, 2020 02 19.
Article en En | MEDLINE | ID: mdl-31929117
Bone is a highly vascularized tissue, in which vascularization and mineralization are concurrent processes during skeletal development. Indeed, both components should be included in any reliable and adherent in vitro model platform for the study of bone physiology and pathogenesis of skeletal disorders. To this end, we developed an in vitro vascularized bone model, using a gelatin-nanohydroxyapatite (gel-nHA) three-dimensional (3D) bioprinted scaffold. First, we seeded human mesenchymal stem cells (hMSCs) on the scaffold, which underwent osteogenic differentiation for 2 weeks. Then, we included lentiviral-GFP transfected human umbilical vein endothelial cells (HUVECs) within the 3D bioprinted scaffold macropores to form a capillary-like network during 2 more weeks of culture. We tested three experimental conditions: condition 1, bone constructs with HUVECs cultured in 1:1 osteogenic medium (OM): endothelial medium (EM); condition 2, bone constructs without HUVECs cultured in 1:1 OM:EM; condition 3: bone construct with HUVECs cultured in 1:1 growth medium:EM. All samples resulted in engineered bone matrix. In conditions 1 and 3, HUVECs formed tubular structures within the bone constructs, with the assembly of a complex capillary-like network visible by fluorescence microscopy in the live tissue and histology. CD31 immunostaining confirmed significant vascular lumen formation. Quantitative real-time PCR was used to quantify osteogenic differentiation and endothelial response. Alkaline phosphatase and runt-related transcription factor 2 upregulation confirmed early osteogenic commitment of hMSCs. Even when OM was removed under condition 3, we observed clear osteogenesis, which was notably accompanied by upregulation of osteopontin, vascular endothelial growth factor, and collagen type I. These findings indicate that we have successfully realized a bone model with robust vascularization in just 4 weeks of culture and we highlighted how the inclusion of endothelial cells more realistically supports osteogenesis. The approach reported here resulted in a biologically inspired in vitro model of bone vascularization, simulating de novo morphogenesis of capillary vessels occurring during tissue development.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Huesos / Ingeniería de Tejidos / Células Madre Mesenquimatosas / Células Endoteliales de la Vena Umbilical Humana Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Huesos / Ingeniería de Tejidos / Células Madre Mesenquimatosas / Células Endoteliales de la Vena Umbilical Humana Tipo de estudio: Evaluation_studies / Prognostic_studies Límite: Humans Idioma: En Revista: Biofabrication Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido