Your browser doesn't support javascript.
loading
Nanoscale subcellular architecture revealed by multicolor three-dimensional salvaged fluorescence imaging.
Zhang, Yongdeng; Schroeder, Lena K; Lessard, Mark D; Kidd, Phylicia; Chung, Jeeyun; Song, Yuanbin; Benedetti, Lorena; Li, Yiming; Ries, Jonas; Grimm, Jonathan B; Lavis, Luke D; De Camilli, Pietro; Rothman, James E; Baddeley, David; Bewersdorf, Joerg.
Afiliación
  • Zhang Y; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Schroeder LK; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Lessard MD; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Kidd P; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Chung J; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Song Y; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.
  • Benedetti L; Howard Hughes Medical Institute, Yale School of Medicine, New Haven, CT, USA.
  • Li Y; Section of Hematology, Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA.
  • Ries J; Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA.
  • Grimm JB; Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.
  • Lavis LD; Howard Hughes Medical Institute, Yale School of Medicine, New Haven, CT, USA.
  • De Camilli P; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
  • Rothman JE; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
  • Baddeley D; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
  • Bewersdorf J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Nat Methods ; 17(2): 225-231, 2020 02.
Article en En | MEDLINE | ID: mdl-31907447
Combining the molecular specificity of fluorescent probes with three-dimensional imaging at nanoscale resolution is critical for investigating the spatial organization and interactions of cellular organelles and protein complexes. We present a 4Pi single-molecule switching super-resolution microscope that enables ratiometric multicolor imaging of mammalian cells at 5-10-nm localization precision in three dimensions using 'salvaged fluorescence'. Imaging two or three fluorophores simultaneously, we show fluorescence images that resolve the highly convoluted Golgi apparatus and the close contacts between the endoplasmic reticulum and the plasma membrane, structures that have traditionally been the imaging realm of electron microscopy. The salvaged fluorescence approach is equally applicable in most single-objective microscopes.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fracciones Subcelulares / Imagen Óptica Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fracciones Subcelulares / Imagen Óptica Límite: Animals / Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos