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Guidelines for panel design, optimization, and performance of whole blood multi-color flow cytometry of platelet surface markers.
Busuttil-Crellin, Xavier; McCafferty, Conor; Van Den Helm, Suelyn; Yaw, Hui Ping; Monagle, Paul; Linden, Matthew; Ignjatovic, Vera.
Afiliación
  • Busuttil-Crellin X; Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia.
  • McCafferty C; Department of Paediatrics, The University of Melbourne , Melbourne, Australia.
  • Van Den Helm S; Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia.
  • Yaw HP; Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia.
  • Monagle P; Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia.
  • Linden M; Department of Paediatrics, The University of Melbourne , Melbourne, Australia.
  • Ignjatovic V; Haematology Research Laboratory, Murdoch Children's Research Institute , Melbourne, Australia.
Platelets ; 31(7): 845-852, 2020 Oct 02.
Article en En | MEDLINE | ID: mdl-31906818
Flow cytometry is a valuable tool in determining the phenotype and function of platelets accurately. The emergence of platelet flow cytometry in recent years provides an attractive alternative to other platelet analytical techniques, with advantages such as requiring small volumes and being highly sensitive to minimal changes in receptor function and expression. Here we present a methodical approach encompassing the stages in the development and optimization of platelet flow cytometry panels based on our extensive experience in this area.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Activación Plaquetaria / Citometría de Flujo Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Platelets Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaquetas / Activación Plaquetaria / Citometría de Flujo Tipo de estudio: Guideline Límite: Humans Idioma: En Revista: Platelets Asunto de la revista: HEMATOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Reino Unido