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The Arabidopsis UDP-glycosyltransferase75B1, conjugates abscisic acid and affects plant response to abiotic stresses.
Chen, Ting-Ting; Liu, Fang-Fei; Xiao, Dong-Wang; Jiang, Xiao-Yi; Li, Pan; Zhao, Shu-Man; Hou, Bing-Kai; Li, Yan-Jie.
Afiliación
  • Chen TT; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Liu FF; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Xiao DW; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Jiang XY; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Li P; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Zhao SM; College of Pharmacy, Liaocheng University, Liaocheng, 252000, China.
  • Hou BK; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
  • Li YJ; The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao, 266237, China.
Plant Mol Biol ; 102(4-5): 389-401, 2020 Mar.
Article en En | MEDLINE | ID: mdl-31894456
KEY MESSAGE: This study revealed that the Arabidopsis UGT75B1 plays an important role in modulating ABA activity by glycosylation when confronting stress environments. The cellular ABA content and activity can be tightly controlled in several ways, one of which is glycosylation by family 1 UDP-glycosyltransferases (UGTs). Previous analysis has shown UGT75B1 activity towards ABA in vitro. However, the biological role of UGT75B1 remains to be elucidated. Here, we characterized the function of UGT75B1 in abiotic stress responses via ABA glycosylation. GUS assay and qRT-PCR indicated that UGT75B1 is significantly upregulated by adverse conditions, such as osmotic stress, salinity and ABA. Overexpression of UGT75B1 in Arabidopsis leads to higher seed germination rates and seedling greening rates upon exposure to salt and osmotic stresses. In contrast, the big UGT75B1 overexpression plants are more sensitive under salt and osmotic stresses. Additionally, the UGT75B1 overexpression plants showed larger stomatal aperture and more water loss under drought condition, which can be explained by lower ABA levels examined in UGT75B1 OE plants in response to water deficit conditions. Consistently, UGT75B1 ectopic expression leads to downregulation of many ABA-responsive genes under stress conditions, including ABI3, ABI5 newly germinated seedlings and RD29A, KIN1, AIL1 in big plants. In summary, our results revealed that the Arabidopsis UGT75B1 plays an important role in coping with abiotic stresses via glycosylation of ABA.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Glicosiltransferasas / Arabidopsis / Ácido Abscísico / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Glucosiltransferasas Idioma: En Revista: Plant Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Fisiológico / Glicosiltransferasas / Arabidopsis / Ácido Abscísico / Regulación de la Expresión Génica de las Plantas / Proteínas de Arabidopsis / Glucosiltransferasas Idioma: En Revista: Plant Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BOTANICA Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos