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Evaluation of the interaction between microencapsulated Bifidobacterium BB-12 added in goat's milk Frozen Yogurt and Escherichia coli in the large intestine.
Verruck, Silvani; Barretta, Clarissa; Miotto, Marília; Canella, Maria Helena Machado; de Liz, Gabriela Rodrigues; Maran, Bruna Marchesan; Garcia, Sofia Grechi; da Silveira, Sheila Mello; Vieira, Cleide Rosana Werneck; da Cruz, Adriano Gomes; Prudencio, Elane Schwinden.
Afiliación
  • Verruck S; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • Barretta C; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • Miotto M; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • Canella MHM; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • de Liz GR; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • Maran BM; Department of Chemistry and Food Engineering, Federal University of Santa Catarina, Technological Center, Florianópolis, SC, Brazil.
  • Garcia SG; Department of Chemistry and Food Engineering, Federal University of Santa Catarina, Technological Center, Florianópolis, SC, Brazil.
  • da Silveira SM; Center of Food Science and Technology, Catarinense Federal Institute Concórdia Campus, Concórdia, SC, Brazil.
  • Vieira CRW; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil.
  • da Cruz AG; Department of Food, Federal Institute of Science and Technology of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
  • Prudencio ES; Department of Food Science and Technology, Federal University of Santa Catarina, Agricultural Science Center, Florianópolis, SC, Brazil; Department of Chemistry and Food Engineering, Federal University of Santa Catarina, Technological Center, Florianópolis, SC, Brazil. Electronic address: elane.prud
Food Res Int ; 127: 108690, 2020 01.
Article en En | MEDLINE | ID: mdl-31882106
Goat milk and goat milk and inulin were used as encapsulating agents of Bifidobacterium BB-12 and applied in Frozen Yogurt (GF2 and GF3, respectively) in order to evaluate the antagonistic effect against Escherichia coli. GF1 is a control containing only Escherichia coli. Simulation of gastrointestinal digestion occurred sequentially. Quantification of Bifidobacterium BB-12 was performed using plate counting while E. coli count was compared with quantification by qPCR with viable and nonviable cell differentiation. The Bifidobacterium BB-12 count was <1.0, 9.23 and 9.11 log CFU g-1 for GF1, GF2 and GF3, respectively. In the ascending colon, all samples showed E. coli counts of approximately 5 log CFU g-1 by plate counting and by qPCR. Throughout the transverse (24 h) and descending colon (48 h) samples GF2 and GF3 showed decrease in E. coli number. GF3 showed higher decrease of E. coli in the descending colon because of inulin bifidogenic characteristic. The production of organic acids by bifidobacteria was directly related to the decrease in the E. coli count. In plate counts, E. coli was not detected for the GF3 sample in the descending colon. However, when quantified by qPCR the sample presented amplification that corresponded to 3 log CFU g-1. In this way, it was possible to observe the phenomenon of the viable but not-culturable cells of E. coli. Finally, it is recommended the microcapsule produced with goat milk and the inulin for application in goat milk products, due to the better antagonist effect against E. coli.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yogur / Leche / Escherichia coli / Bifidobacterium animalis / Microbiología de Alimentos / Intestino Grueso Límite: Animals Idioma: En Revista: Food Res Int Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yogur / Leche / Escherichia coli / Bifidobacterium animalis / Microbiología de Alimentos / Intestino Grueso Límite: Animals Idioma: En Revista: Food Res Int Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Canadá