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Targeting of the Dosage-Compensated Male X-Chromosome during Early Drosophila Development.
Rieder, Leila Elizabeth; Jordan, William Thomas; Larschan, Erica Nicole.
Afiliación
  • Rieder LE; Department of Biology, Emory University, Atlanta, GA 30322, USA. Electronic address: leila.rieder@emory.edu.
  • Jordan WT; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, RI 02912, USA.
  • Larschan EN; Department of Molecular Biology, Cellular Biology, and Biochemistry, Brown University, Providence, RI 02912, USA. Electronic address: erica_larschan@brown.edu.
Cell Rep ; 29(13): 4268-4275.e2, 2019 12 24.
Article en En | MEDLINE | ID: mdl-31875538
Dosage compensation, which corrects for the imbalance in X-linked gene expression between XX females and XY males, represents a model for how genes are targeted for coordinated regulation. However, the mechanism by which dosage compensation complexes identify the X chromosome during early development remains unknown because of the difficulty of sexing embryos before zygotic transcription using X- or Y-linked reporter transgenes. We used meiotic drive to sex Drosophila embryos before zygotic transcription and ChIP-seq to measure the dynamics of dosage compensation factor targeting. The Drosophila male-specific lethal dosage compensation complex (MSLc) requires the ubiquitous zinc-finger protein chromatin-linked adaptor for MSL proteins (CLAMP) to identify the X chromosome. We observe a multi-stage process in which MSLc first identifies CLAMP binding sites throughout the genome, followed by concentration at the strongest X-linked MSLc sites. We provide insight into the dynamics of binding site recognition by a large transcription complex during early development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Compensación de Dosificación (Genética) / Desarrollo Embrionario / Drosophila melanogaster Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Rep Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromosoma X / Compensación de Dosificación (Genética) / Desarrollo Embrionario / Drosophila melanogaster Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Cell Rep Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos