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PPAR γ/Nnat/NF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation.
Yang, Wenkai; Yuan, Wenjin; Peng, Xinghua; Wang, Meiling; Xiao, Jie; Wu, Cheng; Luo, Lie.
Afiliación
  • Yang W; Department of Cardiovascular Surgery, Affiliated Central People's Hospital of Zhanjiang of Guangdong Medical University, Zhanjiang 524045, China.
  • Yuan W; Department of Cardiovascular Medicine, Ganzhou People's Hospital, Ganzhou 341000, China.
  • Peng X; Department of Cardio-Thoracic Surgery, Ganzhou People's Hospital, Ganzhou 341000, China.
  • Wang M; Department of Cardio-Thoracic Surgery, Ganzhou People's Hospital, Ganzhou 341000, China.
  • Xiao J; Department of Cardio-Thoracic Surgery, Ganzhou People's Hospital, Ganzhou 341000, China.
  • Wu C; Department of Cardio-Thoracic Surgery, Ganzhou People's Hospital, Ganzhou 341000, China.
  • Luo L; Department of Cardio-Thoracic Surgery, Ganzhou People's Hospital, Ganzhou 341000, China.
Mediators Inflamm ; 2019: 5618023, 2019.
Article en En | MEDLINE | ID: mdl-31871428
A previous study has demonstrated that adiponectin (APN) could promote preadipocyte differentiation, and the present study further explored its mechanism. 3T3-L1 cells were infected with adenovirus holding human adiponectin gene apM1 and mouse neuronatin (Nnat) shRNA and initiated differentiation while coculturing with mature adipocytes stimulated with LPS. After 8 days, preadipocyte differentiation was observed by Oil Red O staining. Real-time quantitative PCR was used to evaluate mRNA expression levels of monocyte chemoattractant protein-1 (MCP-1), interleukin- (IL-) 6, IL-8, and tumor necrosis factor α (TNF-α). The levels of reactive oxygen species (ROS), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and superoxide dismutase (SOD) in 3T3-L1 cells were detected. Western blotting was done to quantify the protein expression levels of Nnat, peroxisome proliferator-activated receptor (PPAR) γ, p65, and inhibitor of nuclear factor κB (IκB) α. Results demonstrated that APN overexpression markedly increased preadipocyte differentiation; inhibited gene expression of MCP-1, IL-6, IL-8, and TNF-α; reduced ROS and MDA release; increased T-AOC and SOD levels; upregulated Nnat, PPAR γ, and IκB α protein expressions; and downregulated p65 protein expression under LPS stimulation. However, the effects of APN were markedly attenuated when Nnat expression was knocked down. Taken together, the present study provided evidences that the effects of APN on promoting preadipocyte differentiation under inflammatory conditions via anti-inflammation and antioxidative stress may be regulated by the PPAR γ/Nnat/NF-κB signaling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: FN-kappa B / Adipocitos / PPAR gamma / Adiponectina / Proteínas de la Membrana / Proteínas del Tejido Nervioso Límite: Animals / Humans Idioma: En Revista: Mediators Inflamm Asunto de la revista: BIOQUIMICA / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: FN-kappa B / Adipocitos / PPAR gamma / Adiponectina / Proteínas de la Membrana / Proteínas del Tejido Nervioso Límite: Animals / Humans Idioma: En Revista: Mediators Inflamm Asunto de la revista: BIOQUIMICA / PATOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos