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Hyperalphalipoproteinemic scavenger receptor BI knockout mice exhibit a disrupted epidermal lipid barrier.
Martins Cardoso, Renata; Creemers, Eline; Absalah, Samira; Hoekstra, Menno; Gooris, Gert S; Bouwstra, Joke A; Van Eck, Miranda.
Afiliación
  • Martins Cardoso R; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: r.martins.cardoso@lacdr.leidenuniv.nl.
  • Creemers E; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands.
  • Absalah S; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: s.absalah@uva.nl.
  • Hoekstra M; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: hoekstra@lacdr.leidenuniv.nl.
  • Gooris GS; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: gooris_g@lacdr.leidenuniv.nl.
  • Bouwstra JA; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: bouwstra@lacdr.leidenuniv.nl.
  • Van Eck M; Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, Zuid-Holland, the Netherlands. Electronic address: m.eck@lacdr.leidenuniv.nl.
Biochim Biophys Acta Mol Cell Biol Lipids ; 1865(3): 158592, 2020 03.
Article en En | MEDLINE | ID: mdl-31863970
Scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesteryl esters (CE) from high-density lipoproteins (HDL). An impaired SR-BI function leads to hyperalphalipoproteinemia with elevated levels of cholesterol transported in the HDL fraction. Accumulation of cholesterol in apolipoprotein B (apoB)-containing lipoproteins has been shown to alter skin lipid composition and barrier function in mice. To investigate whether these hypercholesterolemic effects on the skin also occur in hyperalphalipoproteinemia, we compared skins of wild-type and SR-BI knockout (SR-BI-/-) mice. SR-BI deficiency did not affect the epidermal cholesterol content and induced only minor changes in the ceramide subclasses. The epidermal free fatty acid (FFA) pool was, however, enriched in short and unsaturated chains. Plasma CE levels strongly correlated with epidermal FFA C18:1 content. The increase in epidermal FFA coincided with downregulation of cholesterol and FFA synthesis genes, suggesting a compensatory response to increased flux of plasma cholesterol and FFAs into the skin. Importantly, the SR-BI-/- epidermal lipid barrier showed increased permeability to ethyl-paraminobenzoic acid, indicating an impairment of the barrier function. In conclusion, increased HDL-cholesterol levels in SR-BI-/- mice can alter the epidermal lipid composition and lipid barrier function similarly as observed in hypercholesterolemia due to elevated levels of apoB-containing lipoproteins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Epidermis / Proteínas de Transferencia de Ésteres de Colesterol / Errores Innatos del Metabolismo Lipídico Límite: Animals Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Epidermis / Proteínas de Transferencia de Ésteres de Colesterol / Errores Innatos del Metabolismo Lipídico Límite: Animals Idioma: En Revista: Biochim Biophys Acta Mol Cell Biol Lipids Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos