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Transcriptome analysis-identified long noncoding RNA CRNDE in maintaining endothelial cell proliferation, migration, and tube formation.
Moran, Matthew; Cheng, Xiao; Shihabudeen Haider Ali, Mohamed Sham; Wase, Nishikant; Nguyen, Nghi; Yang, Weilong; Zhang, Chi; DiRusso, Concetta; Sun, Xinghui.
Afiliación
  • Moran M; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Cheng X; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Shihabudeen Haider Ali MS; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Wase N; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Nguyen N; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Yang W; Center for Plant Science Innovation, School of Biological Sciences, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Zhang C; Center for Plant Science Innovation, School of Biological Sciences, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • DiRusso C; Department of Biochemistry, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
  • Sun X; Nebraska Center for Integrated Biomolecular Communication, University of Nebraska - Lincoln, Lincoln, Nebraska, 68588, USA.
Sci Rep ; 9(1): 19548, 2019 12 20.
Article en En | MEDLINE | ID: mdl-31863035
Obesity is a leading risk factor for type-2 diabetes. Diabetes often leads to the dysregulation of angiogenesis, although the mechanism is not fully understood. Previously, long noncoding RNAs (lncRNAs) have been found to modulate angiogenesis. In this study, we asked how the expression levels of lncRNAs change in endothelial cells in response to excessive palmitic acid treatment, an obesity-like condition. Bioinformatics analysis revealed that 305 protein-coding transcripts were upregulated and 70 were downregulated, while 64 lncRNAs were upregulated and 46 were downregulated. Gene ontology and pathway analysis identified endoplasmic reticulum stress, HIF-1 signaling, and Toll-like receptor signaling as enriched after palmitic acid treatment. Moreover, we newly report enrichment of AGE-RAGE signaling pathway in diabetic complications, IL-17 signaling, and cysteine and methionine metabolism by palmitic acid. One lncRNA, Colorectal Neoplasia Differentially Expressed (CRNDE), was selected for further investigation. Palmitic acid induces CRNDE expression by 1.9-fold. We observed that CRNDE knockdown decreases endothelial cell proliferation, migration, and capillary tube formation. These decreases are synergistic under palmitic acid stress. These data demonstrated that lncRNA CRNDE is a regulator of endothelial cell proliferation, migration, and tube formation in response to palmitic acid, and a potential target for therapies treating the complications of obesity-induced diabetes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Células Endoteliales / ARN Largo no Codificante Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: MicroARNs / Células Endoteliales / ARN Largo no Codificante Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido