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Multiple Sclerosis CD49d+CD154+ As Myelin-Specific Lymphocytes Induced During Remyelination.
Piatek, Pawel; Namiecinska, Magdalena; Domowicz, Malgorzata; Wieczorek, Marek; Michlewska, Sylwia; Matysiak, Mariola; Lewkowicz, Natalia; Tarkowski, Maciej; Lewkowicz, Przemyslaw.
Afiliación
  • Piatek P; Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Poland, ul. Pomorska 251, 92-213 Lodz, Poland.
  • Namiecinska M; Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Poland, ul. Pomorska 251, 92-213 Lodz, Poland.
  • Domowicz M; Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Poland, ul. Pomorska 251, 92-213 Lodz, Poland.
  • Wieczorek M; Department of Neurobiology, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
  • Michlewska S; Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland.
  • Matysiak M; Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Poland, ul. Pomorska 251, 92-213 Lodz, Poland.
  • Lewkowicz N; Department of General Dentistry, Medical University of Lodz, 92-213 Lodz, Poland.
  • Tarkowski M; Department of Biomedical and Clinical Sciences, Luigi Sacco, University Hospital, University of Milan, 20122 Milano, Italy.
  • Lewkowicz P; Department of Neurology, Laboratory of Neuroimmunology, Medical University of Lodz, Poland, ul. Pomorska 251, 92-213 Lodz, Poland.
Cells ; 9(1)2019 12 19.
Article en En | MEDLINE | ID: mdl-31861635
Multiple sclerosis (MS) is a demyelinating autoimmune disease of the central nervous system (CNS) mediated by autoreactive lymphocytes. The role of autoreactive lymphocytes in the CNS demyelination is well described, whereas very little is known about their role in remyelination during MS remission. In this study, we identified a new subpopulation of myelin-specific CD49d+CD154+ lymphocytes presented in the peripheral blood of MS patients during remission, that proliferated in vitro in response to myelin peptides. These lymphocytes possessed the unique ability to migrate towards maturing oligodendrocyte precursor cells (OPCs) and synthetize proinflammatory chemokines/cytokines. The co-culture of maturing OPCs with myelin-specific CD49d+CD154+ lymphocytes was characterized by the increase in proinflammatory chemokine/cytokine secretion that was not only a result of their cumulative effect of what OPCs and CD49d+CD154+ lymphocytes produced alone. Moreover, maturing OPCs exposed to exogenous myelin peptides managed to induce CD40-CD154-dependent CD49d+CD154+ lymphocyte proliferation. We confirmed, in vivo, the presence of CD49d+CD154+ cells close to maturating OPCs and remyelinating plaque during disease remission in the MS mouse model (C57Bl/6 mice immunized with MOG35-55) by immunohistochemistry. Three weeks after an acute phase of experimental autoimmune encephalomyelitis, CD49d+/CD154+ cells were found to be co-localized with O4+ cells (oligodendrocyte progenitors) in the areas of remyelination identified by myelin basic protein (MBP) labelling. These data suggested that myelin-specific CD49d+CD154+ lymphocytes present in the brain can interfere with remyelination mediated by oligodendrocytes probably as a result of establishing proinflammatory environment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ligando de CD40 / Integrina alfa4 / Encefalomielitis Autoinmune Experimental / Esclerosis Múltiple / Vaina de Mielina Tipo de estudio: Observational_studies / Prognostic_studies Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Cells Año: 2019 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ligando de CD40 / Integrina alfa4 / Encefalomielitis Autoinmune Experimental / Esclerosis Múltiple / Vaina de Mielina Tipo de estudio: Observational_studies / Prognostic_studies Límite: Adult / Animals / Female / Humans / Male Idioma: En Revista: Cells Año: 2019 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Suiza