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Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies.
Ou, Yu-Chuan; Wen, Xiaona; Johnson, Christopher A; Shae, Daniel; Ayala, Oscar D; Webb, Joseph A; Lin, Eugene C; DeLapp, Rossane C; Boyd, Kelli L; Richmond, Ann; Mahadevan-Jansen, Anita; Rafat, Marjan; Wilson, John T; Balko, Justin M; Tantawy, Mohammed N; Vilgelm, Anna E; Bardhan, Rizia.
Afiliación
  • Ou YC; Department of Chemical and Biomolecular Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Wen X; Department of Chemical and Biomolecular Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Johnson CA; Department of Veterans Affairs , Tennessee Valley Healthcare System , Nashville , Tennessee 37212 , United States.
  • Shae D; Department of Pharmacology , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
  • Ayala OD; Department of Chemical and Biomolecular Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Webb JA; Department of Biomedical Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Lin EC; Department of Chemical and Biomolecular Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • DeLapp RC; Radiology and Radiological Sciences , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
  • Boyd KL; Vanderbilt University Institute of Imaging Science , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
  • Richmond A; Department of Chemistry and Biochemistry , National Chung Cheng University , Chiayi 62106 , Taiwan.
  • Mahadevan-Jansen A; Civil and Environmental Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Rafat M; Department of Pathology, Microbiology and Immunology , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
  • Wilson JT; Department of Veterans Affairs , Tennessee Valley Healthcare System , Nashville , Tennessee 37212 , United States.
  • Balko JM; Department of Pharmacology , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
  • Tantawy MN; Department of Biomedical Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Vilgelm AE; Department of Chemical and Biomolecular Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
  • Bardhan R; Department of Biomedical Engineering , Vanderbilt University , Nashville , Tennessee 37235 , United States.
ACS Nano ; 14(1): 651-663, 2020 01 28.
Article en En | MEDLINE | ID: mdl-31851488
The overexpression of immunomarker programmed cell death protein 1 (PD-1) and engagement of PD-1 to its ligand, PD-L1, are involved in the functional impairment of cluster of differentiation 8+ (CD8+) T cells, contributing to cancer progression. However, heterogeneities in PD-L1 expression and variabilities in biopsy-based assays render current approaches inaccurate in predicting PD-L1 status. Therefore, PD-L1 screening alone is not predictive of patient response to treatment, which motivates us to simultaneously detect multiple immunomarkers engaged in immune modulation. Here, we have developed multimodal probes, immunoactive gold nanostars (IGNs), that accurately detect PD-L1+ tumor cells and CD8+ T cells simultaneously in vivo, surpassing the limitations of current immunoimaging techniques. IGNs integrate the whole-body imaging of positron emission tomography with high sensitivity and multiplexing of Raman spectroscopy, enabling the dynamic tracking of both immunomarkers. IGNs also monitor response to immunotherapies in mice treated with combinatorial PD-L1 and CD137 agonists and distinguish responders from those nonresponsive to treatment. Our results showed a multifunctional nanoscale probe with capabilities that cannot be achieved with either modality alone, allowing multiplexed immunologic tumor profiling critical for predicting early response to immunotherapies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Nanopartículas del Metal / Imagen Óptica / Oro / Inmunoterapia / Melanoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: ACS Nano Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Biomarcadores de Tumor / Nanopartículas del Metal / Imagen Óptica / Oro / Inmunoterapia / Melanoma Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: ACS Nano Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos