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Loss of Dmrt5 Affects the Formation of the Subplate and Early Corticogenesis.
Ratié, Leslie; Desmaris, Elodie; García-Moreno, Fernando; Hoerder-Suabedissen, Anna; Kelman, Alexandra; Theil, Thomas; Bellefroid, Eric J; Molnár, Zoltán.
Afiliación
  • Ratié L; ULB Neuroscience Institute, Université Libre de Bruxelles, B-6041 Gosselies, Belgium.
  • Desmaris E; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.
  • García-Moreno F; ULB Neuroscience Institute, Université Libre de Bruxelles, B-6041 Gosselies, Belgium.
  • Hoerder-Suabedissen A; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.
  • Kelman A; Achucarro Basque Center for Neuroscience, Parque Científico UPV/EHU Edif. Sede, E-48940 Leioa, Spain.
  • Theil T; IKERBASQUE Foundation, 48013 Bilbao, Spain.
  • Bellefroid EJ; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK.
  • Molnár Z; Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh EH8 9XD, UK.
Cereb Cortex ; 30(5): 3296-3312, 2020 05 14.
Article en En | MEDLINE | ID: mdl-31845734
Dmrt5 (Dmrta2) and Dmrt3 are key regulators of cortical patterning and progenitor proliferation and differentiation. In this study, we show an altered apical to intermediate progenitor transition, with a delay in SP neurogenesis and premature birth of Ctip2+ cortical neurons in Dmrt5-/- mice. In addition to the cortical progenitors, DMRT5 protein appears present in postmitotic subplate (SP) and marginal zone neurons together with some migrating cortical neurons. We observed the altered split of preplate and the reduced SP and disturbed radial migration of cortical neurons into cortical plate in Dmrt5-/- brains and demonstrated an increase in the proportion of multipolar cells in primary neuronal cultures from Dmrt5-/- embryonic brains. Dmrt5 affects cortical development with specific time sensitivity that we described in two conditional mice with slightly different deletion time. We only observed a transient SP phenotype at E15.5, but not by E18.5 after early (Dmrt5lox/lox;Emx1Cre), but not late (Dmrt5lox/lox;NestinCre) deletion of Dmrt5. SP was less disturbed in Dmrt5lox/lox;Emx1Cre and Dmrt3-/- brains than in Dmrt5-/- and affects dorsomedial cortex more than lateral and caudal cortex. Our study demonstrates a novel function of Dmrt5 in the regulation of early SP formation and radial cortical neuron migration. SUMMARY STATEMENT: Our study demonstrates a novel function of Dmrt5 in regulating marginal zone and subplate formation and migration of cortical neurons to cortical plate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Movimiento Celular / Neocórtex / Neuronas Límite: Animals Idioma: En Revista: Cereb Cortex Asunto de la revista: CEREBRO Año: 2020 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Movimiento Celular / Neocórtex / Neuronas Límite: Animals Idioma: En Revista: Cereb Cortex Asunto de la revista: CEREBRO Año: 2020 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Estados Unidos