Your browser doesn't support javascript.
loading
The deleterious effects of smoking in bone mineralization and fibrillar matrix composition.
Barbosa, Alexandre Póvoa; Lourenço, Juliana Dias; Junqueira, Jader Joel Machado; Larissa Emidio de França, Silva; Martins, Janaina S; Oliveira Junior, Manoel Carneiro; Begalli, Isadora; Velosa, Ana Paula Pereira; Olivo, Clarice Rosa; Bastos, Thiago Bernardes; Jorgetti, Vanda; Rodolfo de Paula, Vieira; Teodoro, Walcy Rosolia; Lopes, Fernanda D T Q S.
Afiliación
  • Barbosa AP; Rheumatology Division (LIM-17), School of Medicine, University of São Paulo, Sao Paulo, SP, Brazil. Electronic address: ortopedia_barbosa@yahoo.com.br.
  • Lourenço JD; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Junqueira JJM; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Larissa Emidio de França S; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Martins JS; Department of Medicine, Laboratory of Renal Physiopathology (LIM-16), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Oliveira Junior MC; Nove de Julho University (UNINOVE), Laboratory of Pulmonary and Exercise Immunology (LABPEI), São Paulo, SP, Brazil.
  • Begalli I; Rheumatology Division (LIM-17), School of Medicine, University of São Paulo, Sao Paulo, SP, Brazil.
  • Velosa APP; Rheumatology Division (LIM-17), School of Medicine, University of São Paulo, Sao Paulo, SP, Brazil.
  • Olivo CR; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Bastos TB; Rheumatology Division (LIM-17), School of Medicine, University of São Paulo, Sao Paulo, SP, Brazil.
  • Jorgetti V; Department of Medicine, Laboratory of Renal Physiopathology (LIM-16), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
  • Rodolfo de Paula V; Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE), School of Medical Sciences of São José dos Campos Humanitas and Universidade Brasil, São José dos Campos, SP, Brazil.
  • Teodoro WR; Rheumatology Division (LIM-17), School of Medicine, University of São Paulo, Sao Paulo, SP, Brazil.
  • Lopes FDTQS; Department of Medicine, Laboratory of Experimental Therapeutics (LIM-20), School of Medicine, University of São Paulo, São Paulo, SP, Brazil.
Life Sci ; 241: 117132, 2020 Jan 15.
Article en En | MEDLINE | ID: mdl-31837327
INTRODUCTION: This study aimed to verify the effects of cigarette smoke exposure in bone mineralization and fibrillar matrix composition as well as in bone healing after tibial fracture induction. METHODS: C57Bl/6 Mice were assigned according to exposure and surgery: C room air; F room air and tibia open osteotomy; CS cigarette smoke; FCS cigarette smoke and tibia open osteotomy. In order to study fracture healing we performed, under anesthesia, a bone injury through a tibial shaft osteotomy. Bone samples were obtained to evaluate bone histomorphometry, trabecular morphology and volume, trabecular collagen types composition and presence of inflammatory cytokines and growth factors. RESULTS: CS exposure significantly reduced the thickness of bone trabeculae associated with decrease in mineralizing surface and mineral deposition rate, leading a lower bone formation rate and longer mineralization time. Resorption surface and osteoclastic surface were greater in the CS group, attesting increased resorptive action. There was a decrease in type I collagen deposition and genes expression in the CS and FCS groups compared to C group and in contrast there was an increase in type V collagen deposition and genes expression in the CS, FC and FSC groups compared to C group. Also, CS exposure induced a decrease in bone forming cytokines and an increase in inflammatory associated cytokines, and these changes were intensified under fracture conditions. CONCLUSION: Cigarette smoke exposure alters bone matrix composition and worsens bone mineralization, leading to bone fragility by increasing collagen V synthesis and deposition and impairing collagen I fibril forming and assembling. And these deleterious effects contributed to the worsening in fracture healing after tibia osteotomy.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Humo / Tibia / Fracturas de la Tibia / Calcificación Fisiológica / Fumar Cigarrillos Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Humo / Tibia / Fracturas de la Tibia / Calcificación Fisiológica / Fumar Cigarrillos Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Life Sci Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos