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A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart.
Asp, Michaela; Giacomello, Stefania; Larsson, Ludvig; Wu, Chenglin; Fürth, Daniel; Qian, Xiaoyan; Wärdell, Eva; Custodio, Joaquin; Reimegård, Johan; Salmén, Fredrik; Österholm, Cecilia; Ståhl, Patrik L; Sundström, Erik; Åkesson, Elisabet; Bergmann, Olaf; Bienko, Magda; Månsson-Broberg, Agneta; Nilsson, Mats; Sylvén, Christer; Lundeberg, Joakim.
Afiliación
  • Asp M; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Electronic address: michaela.asp@scilifelab.se.
  • Giacomello S; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden; Department of Biochemistry and Biophysics, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Stockholm University, Stockholm, Sweden.
  • Larsson L; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Wu C; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
  • Fürth D; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
  • Qian X; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
  • Wärdell E; Department of Medicine, Karolinska Institutet, Huddinge, Sweden.
  • Custodio J; Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Reimegård J; Department of Cell and Molecular Biology, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Uppsala University, Uppsala, Sweden.
  • Salmén F; Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands.
  • Österholm C; Department of Molecular Medicine and Surgery, Karolinska Institutet, Stockholm, Sweden.
  • Ståhl PL; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Sundström E; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, R&D Unit, Stockholms Sjukhem, Stockholm, Sweden.
  • Åkesson E; Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, R&D Unit, Stockholms Sjukhem, Stockholm, Sweden.
  • Bergmann O; Center for Regenerative Therapies Dresden, TU-Dresden, Dresden, Germany; Karolinska Institutet, Cell and Molecular Biology, Stockholm, Sweden.
  • Bienko M; Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
  • Månsson-Broberg A; Department of Medicine, Karolinska Institutet, Huddinge, Sweden.
  • Nilsson M; Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
  • Sylvén C; Department of Medicine, Karolinska Institutet, Huddinge, Sweden.
  • Lundeberg J; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Electronic address: joakim.lundeberg@scilifelab.se.
Cell ; 179(7): 1647-1660.e19, 2019 Dec 12.
Article en En | MEDLINE | ID: mdl-31835037
The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación del Desarrollo de la Expresión Génica / Miocitos Cardíacos / Análisis de la Célula Individual / Transcriptoma / Corazón Límite: Female / Humans / Male Idioma: En Revista: Cell Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Regulación del Desarrollo de la Expresión Génica / Miocitos Cardíacos / Análisis de la Célula Individual / Transcriptoma / Corazón Límite: Female / Humans / Male Idioma: En Revista: Cell Año: 2019 Tipo del documento: Article Pais de publicación: Estados Unidos