LC-MS quantification of oligonucleotides in biological matrices with SPE or hybridization extraction.
Bioanalysis
; 11(21): 1941-1954, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31829054
Aim: Quantitative LC-MS analysis of oligonucleotides (OGNs) in biological matrices is needed to support candidate selection of new therapeutic OGNs. Methodology & results: A set of 20 single stranded antisense oligonucleotides (ASO) and five siRNAs were extracted from plasma and tissue homogenates. Anion Exchange (AEX) SPE was selected as generic extraction approach, resulting in recoveries from plasma >70%. Extraction from tissue homogenates showed often more variation and lower recoveries. A proof of concept of a novel tailored hybridization extraction is demonstrated for two 16-mer reference OGNs. Conclusion: Two methods for extraction of OGNs were investigated and applied for quantitative analysis. The AEX-SPE is considered a more generic approach preferred when multiple compounds are evaluated. Hybridization extraction has great potential but critical reagents per analyte are needed.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligonucleótidos
/
Espectrometría de Masas
/
Cromatografía Liquida
/
Extracción en Fase Sólida
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2019
Tipo del documento:
Article
País de afiliación:
Bélgica
Pais de publicación:
Reino Unido