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Cellulose cone tip as a sorbent material for multiphase electrical field-assisted extraction of cocaine from saliva and determination by LC-MS/MS.
Sousa, Denise V M; Pereira, Fabiano V; Nascentes, Clésia C; Moreira, Juliane S; Boratto, Victor H M; Orlando, Ricardo M.
Afiliación
  • Sousa DVM; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.
  • Pereira FV; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.
  • Nascentes CC; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.
  • Moreira JS; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.
  • Boratto VHM; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil.
  • Orlando RM; Laboratory of Microfluidics and Separations, LaMS, Department of Chemistry, Federal University of Minas Gerais, Belo Horizonte, 31270-901, Minas Gerais, Brazil. Electronic address: orlandoricardo@hotmail.com.
Talanta ; 208: 120353, 2020 Feb 01.
Article en En | MEDLINE | ID: mdl-31816720
A porous and hydrophilic sorbent material was used in an extraction system, assisted by electric fields, for the extraction of cocaine in saliva and subsequent determination by ultra-high-performance liquid chromatography associated with sequential triple quadrupole mass spectrometry (UHPLC-MS/MS). The cellulose-based material was characterized by scanning electron microscopy, infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction. The time and voltage variables applied in the extraction process were investigated through a Doehlert experimental design, and with the best conditions found (35min and 300 V) some validation parameters were evaluated. The established working range was 1-100 µg L-1 (R2 > 0.99), and the detection and quantification limits determined were 0.3 and 0.8 µg L-1, respectively. Recoveries from 80 to 115% and coefficient of variation ≤15 and 16% for intra-day and inter-day assays, respectively, were obtained for sample concentrations of LOQ, 5, 25, and 75 µg L-1, indicating satisfactory accuracy and precision for the proposed method. In addition, the method presented no matrix effect, and the extraction efficiency was between 56 and 70%. The results showed that the material used has adequate physicochemical characteristics and can be applied as a sorbent and electrolyte support in multiphase extractions using electric fields.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Celulosa / Cromatografía Líquida de Alta Presión / Cocaína / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Límite: Humans Idioma: En Revista: Talanta Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saliva / Celulosa / Cromatografía Líquida de Alta Presión / Cocaína / Espectrometría de Masas en Tándem / Extracción en Fase Sólida Límite: Humans Idioma: En Revista: Talanta Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos