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Effect of particle functionalization and solution properties on the adsorption of bovine serum albumin and lysozyme onto silica nanoparticles.
Galdino, Flávia Elisa; Picco, Agustin Silvio; Sforca, Maurício Luis; Cardoso, Mateus Borba; Loh, Watson.
Afiliación
  • Galdino FE; Institute of Chemistry, University of Campinas (UNICAMP), CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil; Brazilian Nanotechnology National Laboratory (LNNano), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil.
  • Picco AS; Brazilian Nanotechnology National Laboratory (LNNano), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil.
  • Sforca ML; Brazilian Biosciences Laboratory (LNBio), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil.
  • Cardoso MB; Institute of Chemistry, University of Campinas (UNICAMP), CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil; Brazilian Nanotechnology National Laboratory (LNNano), National Center for Research in Energy and Materials (CNPEM) CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil. Electronic address:
  • Loh W; Institute of Chemistry, University of Campinas (UNICAMP), CP 6154, CEP 13083-970, Campinas, São Paulo, Brazil. Electronic address: wloh@unicamp.br.
Colloids Surf B Biointerfaces ; 186: 110677, 2020 Feb.
Article en En | MEDLINE | ID: mdl-31812075
Silica nanoparticles present an enormous potential as controlled drug delivery systems with high selectivity towards diseased cells. This application is directly related to the phenomenon of protein corona, characterized by the spontaneous adsorption of proteins on the nanoparticle surface, which is not fully understood. Here, we report an investigation on the influence of pH, ionic strength and temperature on the thermodynamics of interaction of bovine serum albumin protein (BSA) with non-functionalized silica nanoparticles (SiO2NPs). Complementary, we also investigated the ability of polyethylene glycol (PEG) and zwitterionic sulfobetaine (SBS) surface-modified nanoparticles to prevent the adsorption of BSA (protein negatively charged at physiological pH) and lysozyme (protein positively charged at physiological pH). We showed that BSA interaction with SiO2NPs is enthalpically governed. On the other hand, functionalization of silica nanoparticles with PEG and SBS completely prevented BSA adsorption. However, these functionalized nanoparticles presented a negative zeta potential and were not able to suppress lysozyme anchoring due to strong nanoparticle-protein electrostatic attraction. Due to the similarity of BSA with Human Serum Albumin, this investigation bears a resemblance to processes involved in the phenomenon of protein corona in human blood, producing information that is relevant for the future biomedical use of functionalized nanoparticles.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Muramidasa / Dióxido de Silicio / Nanopartículas Límite: Animals Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Albúmina Sérica Bovina / Muramidasa / Dióxido de Silicio / Nanopartículas Límite: Animals Idioma: En Revista: Colloids Surf B Biointerfaces Asunto de la revista: QUIMICA Año: 2020 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos