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Three-dimensional electron microscopy analysis reveals endopolygeny-like nuclear architecture segregation in Plasmodium oocyst development.
Araki, Tamasa; Kawai, Satoru; Kakuta, Soichiro; Kobayashi, Hirotaka; Umeki, Yuko; Saito-Nakano, Yumiko; Sasaki, Toshinori; Nagamune, Kisaburo; Yasutomi, Yasuhiro; Nozaki, Tomoyoshi; Franke-Fayard, Blandine; Khan, Shahid M; Hisaeda, Hajime; Annoura, Takeshi.
Afiliación
  • Araki T; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.
  • Kawai S; Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Mibu, Tochigi, Japan.
  • Kakuta S; Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine, Bunkyo, Tokyo 113-8421, Japan.
  • Kobayashi H; Department of Pathology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
  • Umeki Y; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
  • Saito-Nakano Y; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
  • Sasaki T; Department of Medical Entomology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo 162-8640, Japan.
  • Nagamune K; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
  • Yasutomi Y; Laboratory of Immunoregulation and Vaccine Research, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Health and Nutrition, 1-1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan.
  • Nozaki T; Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
  • Franke-Fayard B; Leiden Malaria Research Group, Parasitology, Center of Infectious Diseases, Leiden University Medical Center (LUMC), 2333 ZA Leiden, the Netherlands.
  • Khan SM; Leiden Malaria Research Group, Parasitology, Center of Infectious Diseases, Leiden University Medical Center (LUMC), 2333 ZA Leiden, the Netherlands.
  • Hisaeda H; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
  • Annoura T; Department of Parasitology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Electronic address: annoura@niid.go.jp.
Parasitol Int ; 76: 102034, 2020 Jun.
Article en En | MEDLINE | ID: mdl-31805442
The genus Plasmodium is a unicellular eukaryotic parasite that is the causative agent of malaria, which is transmitted by Anopheline mosquito. There are a total of three developmental stages in the production of haploid parasites in the Plasmodium life cycle: the oocyst stage in mosquitoes and the liver and blood stages in mammalian hosts. The Plasmodium oocyst stage plays an important role in the production of the first generation of haploid parasites. Nuclear division is the most important event that occurs during the proliferation of all eukaryotes. However, obtaining the details of nuclear division at the oocyst stage is challenging owing to difficulties in preparation. In this study, we used focused-ion-beam-milling combined with scanning-electron-microscopy to report the 3D architecture during nuclear segregations in oocyst stage. This advanced technology allowed us to analyse the 3D details of organelle segregation inside the oocyst during sporogony formation. It was revealed that multiple nuclei were involved with several centrosomes in one germ nucleus during sporozoite budding (endopolygeny). Our high-resolution 3D analysis uncovered the endopolygeny-like nuclear architecture of Plasmodium in the definitive host. This nuclear segregation was different from that in the blood stage, and its similarity to other apicomplexan parasite nuclear divisions such as Sarcocystis is discussed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium / Núcleo Celular / Oocistos / División del Núcleo Celular Límite: Animals Idioma: En Revista: Parasitol Int Asunto de la revista: PARASITOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasmodium / Núcleo Celular / Oocistos / División del Núcleo Celular Límite: Animals Idioma: En Revista: Parasitol Int Asunto de la revista: PARASITOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos