DNA-binding proteins in protein kinase C preparations.

Testori, A; Hii, C S; Fournier, A; Burgoyne, L A; Murray, A W

Testori, A; Hii, C S; Fournier, A; Burgoyne, L A; Murray, A W.

Afiliación

Testori A; School of Biological Sciences, Flinders University, Bedford Park, South Australia.

Biochem Biophys Res Commun ; 156(1): 222-7, 1988 Oct 14.

Article en En | MEDLINE | ID: mdl-3178832

RESUMEN

Human DNA enriched in repetitive sequences specifically bound to a component(s) in purified preparations of rat brain protein kinase C (PKC). DNA which bound to protein was cloned in pUC-19 and one clone characterized as containing an approx. 140 bp insert. The band containing this insert (separated by acrylamide gel electrophoresis) was lost when the DNA was incubated with purified PKC preparations. Thus a protein in relatively pure PKC preparations is a sequence-selective DNA-binding protein. The results raise the possibility that PKC or a fragment of PKC binds selectively to specific DNA sequences.

Asunto(s)

Encéfalo/metabolismo; Proteínas de Unión al ADN/aislamiento & purificación; ADN/genética; Proteína Quinasa C/aislamiento & purificación; Animales; Clonación Molecular; ADN/metabolismo; Proteínas de Unión al ADN/inmunología; Proteínas de Unión al ADN/metabolismo; Humanos; Sueros Inmunes; Peso Molecular; Proteína Quinasa C/metabolismo; Ratas; Secuencias Repetitivas de Ácidos Nucleicos

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Proteína Quinasa C / ADN / Proteínas de Unión al ADN Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 1988 Tipo del documento: Article Pais de publicación: Estados Unidos

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