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Zwitterionic HILIC stationary phase as a valuable alternative in separative techniques: Application to the analysis of gamma-hydroxybutyric acid and its metabolite in hair.
Pascali, Jennifer P; Fais, Paolo; Vaiano, Fabio; Ciolini, Alice; Bertol, Elisabetta.
Afiliación
  • Pascali JP; Forensic Toxicology Division, Department of Health Sciences, University of Florence, Largo Brambilla 3, Florence 50134, Italy. Electronic address: jennifer.pascali@gmail.com.
  • Fais P; University of Bologna, Department of Medical and Surgical Sciences, Unit of Legal Medicine, Via Irnerio 49, Bologna, Italy.
  • Vaiano F; Forensic Toxicology Division, Department of Health Sciences, University of Florence, Largo Brambilla 3, Florence 50134, Italy.
  • Ciolini A; Forensic Toxicology Division, Department of Health Sciences, University of Florence, Largo Brambilla 3, Florence 50134, Italy.
  • Bertol E; Forensic Toxicology Division, Department of Health Sciences, University of Florence, Largo Brambilla 3, Florence 50134, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1134-1135: 121876, 2019 Dec 15.
Article en En | MEDLINE | ID: mdl-31783252
In this work, the physical and chemical properties of a novel zwitterionic LC stationary phase are applied to the development, validation and application of a new fast and reliable method devoted to the analysis of GHB (gamma-hydroxybutyric acid) and its relatively new discovered glucuronide metabolite in hair. The obtained sensitivity, expressed as limit of detection (LOD) and quantification (LOQ), were 0.033 and 0.10 ng/mg for GHB and 0.11 and 0.37 ng/mg, for GHB-glucuronide respectively. Linearity was assessed between LOQ and 50 ng/mg for both compounds. GHB and GHB-glucuronide extraction from hair matrix was maintained simple and consisted in an acidified-solvent incubation. No samples purification was required before LC-MS/MS analysis. The method was finally applied to 65 real hair sample, 60 adults and 5 children below 2 years old. The obtained results highlighted that GHB concentrations were in the range 0.11-0.96 ng/mg (average 0.38 ±â€¯0.25 ng/mg) in 44 cases (68%) while in 21 samples GHB concentrations were in the range between LOD and LOQ (0.033-0.1 ng/mg). GHB-glucuronide was detected in few samples (n. 3) at levels below LOQ. The interest on these molecules relies on the fact that GHB is both a naturally occurring inhibitory neurotransmitter in the central nervous system and an illicit drug often experienced by victims of drug-facilitated sexual assault. GHB-glucuronide was firstly identified in urine by the group of Petersen in 2013 and, as per analogy to ethyl glucuronide, it was proposed as a longer biomarker for GHB intoxication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Cabello / Hidroxibutiratos Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Infant / Male Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Cabello / Hidroxibutiratos Tipo de estudio: Prognostic_studies Límite: Adult / Female / Humans / Infant / Male Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos