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Astrovirus Replication Is Inhibited by Nitazoxanide In Vitro and In Vivo.
Hargest, Virginia; Sharp, Bridgett; Livingston, Brandi; Cortez, Valerie; Schultz-Cherry, Stacey.
Afiliación
  • Hargest V; Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
  • Sharp B; Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee, USA.
  • Livingston B; Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
  • Cortez V; Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
  • Schultz-Cherry S; Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee, USA.
J Virol ; 94(5)2020 02 14.
Article en En | MEDLINE | ID: mdl-31776285
Astroviruses (AstV) are a leading cause of diarrhea, especially in the very young, the elderly, and immunocompromised populations. Despite their significant impact on public health, no drug therapies for astrovirus have been identified. In this study, we fill this gap in knowledge and demonstrate that the FDA-approved broad-spectrum anti-infective drug nitazoxanide (NTZ) blocks astrovirus replication in vitro with a 50% effective concentration (EC50) of approximately 1.47 µM. It can be administered up to 8 h postinfection and is effective against multiple human astrovirus serotypes, including clinical isolates. Most importantly, NTZ reduces viral shedding in vivo, exhibiting its potential as a future clinical therapeutic.IMPORTANCE Human astroviruses (HAstV) are thought to cause between 2 and 9% of acute, nonbacterial diarrhea cases in children worldwide. HAstV infection can be especially problematic in immunocompromised people and infants, where the virus has been associated with necrotizing enterocolitis and severe and persistent diarrhea, as well as rare instances of systemic and fatal disease. And yet, no antivirals have been identified to treat astrovirus infection. Our study provides the first evidence that nitazoxanide may be an effective therapeutic strategy against astrovirus disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mamastrovirus / Tiazoles / Replicación Viral / Infecciones por Astroviridae Límite: Animals / Humans Idioma: En Revista: J Virol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mamastrovirus / Tiazoles / Replicación Viral / Infecciones por Astroviridae Límite: Animals / Humans Idioma: En Revista: J Virol Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos