Increased expression of ecotropic viral integration site 2A indicates a poor prognosis and promotes osteosarcoma evolution through activating MEK/ERK pathway.

Li, Shuo; Yang, Feng; Yang, Yao-Kun; Zhou, Yun

Li, Shuo; Yang, Feng; Yang, Yao-Kun; Zhou, Yun.

Afiliación

Li S; Department of Orthopaedics, Suzhou Municipal Hospital, Suzhou, Anhui, P.R. China.
Yang F; Department of Orthopaedics, Suzhou Municipal Hospital, Suzhou, Anhui, P.R. China.
Yang YK; Department of Orthopaedics, Suzhou Municipal Hospital, Suzhou, Anhui, P.R. China.
Zhou Y; Department of Orthopaedics, The Second Hospital of Anhui Medical University, Hefei, Anhui, P.R. China.

J Recept Signal Transduct Res ; 39(4): 368-372, 2019 Aug.

Article en En | MEDLINE | ID: mdl-31774019

RESUMEN

Although ecotropic viral integration site 2 A (EVI2A) plays key roles in several cancers, the expression and function of EVI2A in osteosarcoma (OS) have not been investigated. Hence, we explored the expression of EVI2A and its clinical significance of EVI2A of OS. Firstly, we investigated the expression of EVI2A in OS tissues. The relationship of EVI2A expression and survival time was analyzed using Kaplan-Meier plotter. Then, we used quantitative reverse transcription PCR (qRT-PCR) to confirm the expression level of EVI2A in OS cell lines. Cell proliferation, and wound-healing experiments were used to identify the biological function of EVI2A. Moreover, EVI2A-mediated MEK/ERK signaling pathway was evaluated using western blotting. Data suggested that EVI2A was highly expressed in OS tissues, and high-expression of EVI2A was associated with worse overall survival in OS patients. Moreover, the up-regulation of it was observed in OS cell lines (Saos2, and MG63). Knockdown of EVI2A suppressed cell proliferation and migration of OS. Western blotting revealed that the inactivation of MEK/ERK pathway was found in OS cells after EVI2A knockdown. Our data implicated the crucial role of EVI2A in the progression of OS, demonstrating that expression of EVI2A may offer an attractive novel prognostic signature for OS.

Asunto(s)

Neoplasias Óseas/patología; Regulación Neoplásica de la Expresión Génica; MAP Quinasa Quinasa 1/metabolismo; Proteínas de la Membrana/metabolismo; Proteína Quinasa 1 Activada por Mitógenos/metabolismo; Proteína Quinasa 3 Activada por Mitógenos/metabolismo; Osteosarcoma/patología; Apoptosis; Biomarcadores de Tumor/genética; Biomarcadores de Tumor/metabolismo; Neoplasias Óseas/metabolismo; Movimiento Celular; Proliferación Celular; Progresión de la Enfermedad; Humanos; MAP Quinasa Quinasa 1/genética; Proteínas de la Membrana/genética; Proteína Quinasa 1 Activada por Mitógenos/genética; Proteína Quinasa 3 Activada por Mitógenos/genética; Osteosarcoma/metabolismo; Pronóstico; Transducción de Señal; Células Tumorales Cultivadas

Palabras clave

Osteosarcoma; ecotropic viral integration site 2A; prognosis; proliferation

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Óseas / Osteosarcoma / Regulación Neoplásica de la Expresión Génica / Proteína Quinasa 1 Activada por Mitógenos / MAP Quinasa Quinasa 1 / Proteína Quinasa 3 Activada por Mitógenos / Proteínas de la Membrana Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Recept Signal Transduct Res Asunto de la revista: BIOQUIMICA / FISIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido

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