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Identification of a new GLDC gene alternative splicing variant and its protumorigenic roles in lung cancer.
Yuan, Yingli; Sun, Luguo; Wang, Xu; Chen, Jingxian; Jia, Mingnan; Zou, Yunlong; Sa, Huanlan; Cai, Yangyang; Xu, Yinghui; Sun, Chao; Guo, Ye; Li, Hongwei; Ma, Kewei.
Afiliación
  • Yuan Y; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Sun L; National Engineering Laboratory for Druggable Gene & Protein Screening, Northeast Normal University, Changchun 130024, PR China.
  • Wang X; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Chen J; National Engineering Laboratory for Druggable Gene & Protein Screening, Northeast Normal University, Changchun 130024, PR China.
  • Jia M; Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, PR China.
  • Zou Y; Orthopaedics Surgery Department, China-Japan Union Hospital of Jilin University, Changchun 130033, PR China.
  • Sa H; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Cai Y; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Xu Y; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Sun C; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Guo Y; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Li H; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
  • Ma K; Oncology Center, First Hospital, Jilin University, Changchun 130021, PR China.
Future Oncol ; 15(36): 4127-4139, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31773974
Aim: To clarify the regulatory roles of GLDCV1, the first identified truncated glycine decarboxylase (GLDC), on cancer stem cells and tumorigenesis. Materials & methods: RT-PCR or RT-qPCR, immunoblotting and immunohistochemical staining were applied to assess gene expression. MTT, BrdU incorporation and colony formation assays were used to examine cell proliferation capacity. Soft agar colony formation and in vivo transplantation were applied to evaluate cellular transformation and tumorigenesis. Results & conclusion: Expression of GLDCV1 or GLDC was enhanced in non-small-cell lung cancer cell line and clinical samples. GLDCV1 overexpression induced MRC5 cell proliferation, transformation and tumorigenesis. Additionally, GLDCV1 increased lactate production and cancer stem cell marker expression and activated ERK and P38 pathways. Our study gained deeper insight into GLDC oncogene.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transformación Celular Neoplásica / Empalme Alternativo / Glicina-Deshidrogenasa (Descarboxilante) / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Future Oncol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transformación Celular Neoplásica / Empalme Alternativo / Glicina-Deshidrogenasa (Descarboxilante) / Neoplasias Pulmonares Tipo de estudio: Diagnostic_studies Límite: Animals / Female / Humans Idioma: En Revista: Future Oncol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido