Screening and identification of novel isolate Streptomyces sp., NLKPB45 from Nellore costal region for its biomedical applications.
Saudi J Biol Sci
; 26(7): 1655-1660, 2019 Nov.
Article
en En
| MEDLINE
| ID: mdl-31762640
Actinobacteria, which are the prolific producers of antibiotics and significant suppliers to the pharmaceutical industry, can produce a wide variety of bioactive metabolites. An actinomycete strain designated NLKPB45 was isolated from mangrove soils samples of Nellore coastal regions Andhra Pradesh and assessed for antibiotic production and activity against pathogenic bacteria. From a total of 9 mangrove soil samples, 143 acinomycetes were isolated. Among the isolated them 6 actinomycetes strains showed potential antibacterial activity against at two tested pathogens gram positive and gram negative bacteria E. coli and S. aureus. The potent strain NLKPB45 was identified by 16S gene isolation and sequencing to the Streptomyces genus. The ethyl acetate extracts also as shown excellent antimicrobial activity against Salmonella sp., staphylococcus aureus, E. coli, and B. subtilus were detected in both the supernatant extract samples from fermentations of culture NLKPB45. The anticancer activity of extracts in the HeLa with IC50 value of 37.1924⯵g/ml, MCF-7 IC50 value of 40.9177⯵g/ml and HT 29 IC50 value of 43.3758⯵g/ml.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Saudi J Biol Sci
Año:
2019
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
Arabia Saudita