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Target analysis and retrospective screening of mycotoxins and pharmacologically active substances in milk using an ultra-high-performance liquid chromatography/high-resolution mass spectrometry approach.
Izzo, Luana; Rodríguez-Carrasco, Yelko; Tolosa, Josefa; Graziani, Giulia; Gaspari, Anna; Ritieni, Alberto.
Afiliación
  • Izzo L; Department of Pharmacy, Faculty of Pharmacy, University of Naples "Federico II", Via Domenico Montesano 49, 80131 Napoli, Italy.
  • Rodríguez-Carrasco Y; Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain. Electronic address: yelko.rodriguez@uv.es.
  • Tolosa J; Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain.
  • Graziani G; Department of Pharmacy, Faculty of Pharmacy, University of Naples "Federico II", Via Domenico Montesano 49, 80131 Napoli, Italy.
  • Gaspari A; Department of Pharmacy, Faculty of Pharmacy, University of Naples "Federico II", Via Domenico Montesano 49, 80131 Napoli, Italy.
  • Ritieni A; Department of Pharmacy, Faculty of Pharmacy, University of Naples "Federico II", Via Domenico Montesano 49, 80131 Napoli, Italy.
J Dairy Sci ; 103(2): 1250-1260, 2020 Feb.
Article en En | MEDLINE | ID: mdl-31759606
Milk is a nutritious food suitable for infants and adults, and it plays an important role in the human diet. However, it may also be a vehicle for food contaminants. In this report, we developed a method using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-Q-Exactive Orbitrap HRMS; Thermo Fisher Scientific, Waltham, MA) for simultaneous identification of target pharmacologically active substances and mycotoxins in milk. We also used the Q-Orbitrap operating in full scan mode to identify other possible drugs and microbial metabolites that occurred in samples. Fifty-six commercially available milk samples from the Italian market were analyzed. Investigated analytes were extracted using a QuEChERS (quick, easy, cheap, effective, rugged, and safe) approach. Method detection and quantification limits and performance criteria set by European regulations were fulfilled. Pharmacologically active substances were detected in 49% of samples (range 0.007-4.53 ng/mL), including nontarget mycotoxins. Retrospective analysis allowed us to identify other antibiotics and pharmacologically active substances, as well as nonregulated fungal/bacterial metabolites at a relatively high incidence. From the obtained values, the need for continuous monitoring of contaminants in the milk production chain is clear. This is the first study to assess the presence of pharmacologically active substances, mycotoxins, and other microbial metabolites in Italian milk samples using the UHPLC-Q-Orbitrap HRMS system.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Cromatografía Líquida de Alta Presión / Leche / Micotoxinas Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies / Screening_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: J Dairy Sci Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas / Cromatografía Líquida de Alta Presión / Leche / Micotoxinas Tipo de estudio: Diagnostic_studies / Observational_studies / Prognostic_studies / Screening_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: J Dairy Sci Año: 2020 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos