N-WASP knockdown upregulates inflammatory cytokines expression in human gingival fibroblasts.

Wang, Yijia; Kang, Wenyan; Shang, Lingling; Song, Aimei; Ge, Shaohua

Wang, Yijia; Kang, Wenyan; Shang, Lingling; Song, Aimei; Ge, Shaohua.

Afiliación

Wang Y; Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, 250012, Jinan, Shandong, China
Kang W; Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, 250012, Jinan, Shandong, China
Shang L; Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, 250012, Jinan, Shandong, China
Song A; Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, 250012, Jinan, Shandong, China
Ge S; Department of Periodontology, School and Hospital of Stomatology, Shandong University & Shandong Provincial Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, No.44-1 Wenhua Road West, 250012, Jinan, Shandong, China

Arch Oral Biol ; 110: 104605, 2020 Feb.

Article en En | MEDLINE | ID: mdl-31751919

RESUMEN

OBJECTIVE: The neuronal wiskott-aldrich syndrome protein (N-WASP) is a member of the wiskott-aldrich syndrome protein (WASP) family. N-WASP plays a vital role in promoting cell migration, receptor signaling and immune inflammatory responses. This study aimed to observe the changes in the expression of inflammatory factors and involving pathways after N-WASP knockdown in human gingival fibroblasts (HGFs). DESIGN: Gingival inflammatory condition of N-WASP knockout mice was evaluated by H&E staining. N-WASP in HGFs was knockdown by siRNA and the best knockdown efficiency was determined by qRT-PCR and immunofluorescence. The mRNA levels of interleukin (IL)-6, IL-8, C-C motif ligand 2 (CCL2), superoxide dismutase 2 (SOD2) and prostaglandin endoperoxide synthase 2 (PTGS2) were evaluated by qRT-PCR after N-WASP knockdown with or without mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) inhibitors. The protein levels of IL-6, IL-8 and CCL2 were assessed by ELISA. Western blotting was used to detect the activation of NF-κB and MAPK signaling pathways. RESULTS: Gingival tissue from N-WASP knockout mice exhibited an inflammatory reaction. The expression of IL-6, IL-8, CCL2, SOD2 and PTGS2 was significantly upregulated after N-WASP knockdown in HGFs for 6, 24 and 48â¯h, except for the SOD2 at 6â¯h. N-WASP knockdown significantly activated the signaling pathways of NF-κB and MAPK. The inhibitors of p65, p38, ERK and JNK clearly decreased IL-6, IL-8, CCL2, SOD2 and PTGS2 expression after N-WASP knockdown. CONCLUSION: These data indicated that N-WASP deficiency in HGFs increases the production of inflammatory cytokine and is regulated via NF-κB and MAPK signaling pathways.

Asunto(s)

Citocinas; Fibroblastos; Proteína Neuronal del Síndrome de Wiskott-Aldrich; Animales; Citocinas/metabolismo; Fibroblastos/metabolismo; Técnicas de Silenciamiento del Gen; Encía/metabolismo; Humanos; Ratones; Quinasas de Proteína Quinasa Activadas por Mitógenos/metabolismo; FN-kappa B/metabolismo; Transducción de Señal; Proteína Neuronal del Síndrome de Wiskott-Aldrich/genética; Proteína Neuronal del Síndrome de Wiskott-Aldrich/metabolismo

Palabras clave

Human gingival fibroblasts; Inflammation cytokine; N-WASP; Psoriasis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Citocinas / Proteína Neuronal del Síndrome de Wiskott-Aldrich / Fibroblastos Límite: Animals / Humans Idioma: En Revista: Arch Oral Biol Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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