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Impedimetric and electrochemical evaluation of a new redox active steroid derivative for hormone immunosensing.
Kelch, Jessica; Delaney, Aoife; Kelleher, Fintan; Baker, Priscilla; Iwuoha, Emmanuel; Dempsey, Eithne.
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  • Kelch J; Centre of Applied Science for Health, Technological University Dublin, Tallaght Campus, Dublin 24, Ireland.
  • Delaney A; Centre of Applied Science for Health, Technological University Dublin, Tallaght Campus, Dublin 24, Ireland.
  • Kelleher F; Molecular Design and Synthesis Group, Centre for Applied Science for Health, Technological University Dublin, Tallaght Campus, Dublin 24, Ireland.
  • Baker P; SensorLab, Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.
  • Iwuoha E; SensorLab, Department of Chemistry, University of the Western Cape, Private Bag X17, Bellville, 7535, South Africa.
  • Dempsey E; Department of Chemistry, Maynooth University, Maynooth, Co. Kildare, Ireland. Electronic address: eithne.dempsey@mu.ie.
Biosens Bioelectron ; 150: 111876, 2020 Feb 15.
Article en En | MEDLINE | ID: mdl-31748195
Preparation and electrochemical interrogation of a novel redox active progesterone derivative progesterone thiosemicarbazone (PATC) is presented here together with an investigation into its suitability as conjugate in progesterone hormone immunosensing. PATC synthesis involved a condensation reaction between progesterone acetate and thiosemicarbazone hydrochloride. Voltammetric and pulse techniques confirmed the redox behaviour of the new compound with concentration and scan rate dependant irreversible behaviour evident at glassy carbon and gold transducers - ko (standard heterogeneous rate constant) was 2.56 × 10-3 cm2/s (ν = 100 mV/s in non-aqeuous media). Bioaffinity studies towards anti-progesterone antibodies involved a competitive ELISA format (optical) which confirmed recognition of the new progesterone derivative. Electrochemical impedance spectroscopy was employed as an interrogation technique in order to establish optimum binding and surface conditions for progesterone antigen-antibody interaction with the assistance of a redox probe (potassium hexacyanoferrate).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Técnicas Biosensibles / Anticuerpos Inmovilizados Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Reino Unido
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Progesterona / Técnicas Biosensibles / Anticuerpos Inmovilizados Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article País de afiliación: Irlanda Pais de publicación: Reino Unido