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Evaluation of Carotenoids Protection Against Oxidative Stress in the Animal Model Caenorhabditis elegans.
Martorell, Patricia; Llopis, Silvia; Gil, José Vicente; Genovés, Salvador; Ramón, Daniel; Zacarías, Lorenzo; Rodrigo, María Jesús.
Afiliación
  • Martorell P; Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL/Archer Daniels Midland, Paterna, Valencia, Spain.
  • Llopis S; Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL/Archer Daniels Midland, Paterna, Valencia, Spain.
  • Gil JV; Food Technology Area, Faculty of Pharmacy, University of Valencia, Burjassot, Valencia, Spain.
  • Genovés S; Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain.
  • Ramón D; Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL/Archer Daniels Midland, Paterna, Valencia, Spain.
  • Zacarías L; Cell Biology Laboratory, Food Biotechnology Department, Biópolis SL/Archer Daniels Midland, Paterna, Valencia, Spain.
  • Rodrigo MJ; Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Paterna, Valencia, Spain.
Methods Mol Biol ; 2083: 387-401, 2020.
Article en En | MEDLINE | ID: mdl-31745937
The nematode Caenorhabditis elegans is a versatile and powerful model organism for animal experimental research and, despite being an invertebrate, displays remarkably similar molecular bases and conserved cellular pathways to those of humans. Oxidative stress is an etiological factor that influences numerous diseases, degenerative processes and aging. C. elegans has revealed as an opportune and feasible organism to investigate the antioxidant effects of different bioactives or complex food matrices, and a number of protocols have been developed by using different oxidative stressors. Carotenoids are recognized as quenchers and scavengers of reactive oxygen species, and many of their related health benefits attributed in the diet are tightly linked to their antioxidant properties. In this chapter, we report a simple and rapid assay to evaluate the protection capacity of pure carotenoids or complex carotenoid extracts against oxidative stress in the model system C. elegans. The protocol describes a representative feeding experiment by adding carotenoids to the nematode growth medium and after an incubation period, the C. elegans populations fed with carotenoids are exposed to an acute oxidative stress by using H2O2 as oxidative agent. The protection against oxidative stress is evaluated as the survival rate of the nematodes fed with the carotenoid prior to receiving oxidative treatment compared with the survival rate of control nematode population. In order to confirm the carotenoid intake by the nematodes during the feeding experiment a bioassimilation experiment is also reported.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Caenorhabditis elegans / Estrés Oxidativo / Antioxidantes Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Carotenoides / Caenorhabditis elegans / Estrés Oxidativo / Antioxidantes Tipo de estudio: Guideline / Prognostic_studies Límite: Animals Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Estados Unidos