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Embryonic progenitor pools generate diversity in fine-scale excitatory cortical subnetworks.
Ellender, Tommas J; Avery, Sophie V; Mahfooz, Kashif; Scaber, Jakub; von Klemperer, Alexander; Nixon, Sophie L; Buchan, Matthew J; van Rheede, Joram J; Gatti, Aleksandra; Waites, Cameron; Pavlou, Hania J; Sims, David; Newey, Sarah E; Akerman, Colin J.
Afiliación
  • Ellender TJ; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Avery SV; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Mahfooz K; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Scaber J; MRC Computational Genomics Analysis and Training Programme (CGAT), MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Oxford, OX3 9DS, UK.
  • von Klemperer A; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Nixon SL; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Buchan MJ; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • van Rheede JJ; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Gatti A; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Waites C; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Pavlou HJ; MRC Computational Genomics Analysis and Training Programme (CGAT), MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Oxford, OX3 9DS, UK.
  • Sims D; MRC Computational Genomics Analysis and Training Programme (CGAT), MRC WIMM Centre for Computational Biology, MRC Weatherall Institute of Molecular Medicine, Oxford, OX3 9DS, UK.
  • Newey SE; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK.
  • Akerman CJ; Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, UK. colin.akerman@pharm.ox.ac.uk.
Nat Commun ; 10(1): 5224, 2019 11 19.
Article en En | MEDLINE | ID: mdl-31745093
The mammalian neocortex is characterized by a variety of neuronal cell types and precise arrangements of synaptic connections, but the processes that generate this diversity are poorly understood. Here we examine how a pool of embryonic progenitor cells consisting of apical intermediate progenitors (aIPs) contribute to diversity within the upper layers of mouse cortex. In utero labeling combined with single-cell RNA-sequencing reveals that aIPs can generate transcriptionally defined glutamatergic cell types, when compared to neighboring neurons born from other embryonic progenitor pools. Whilst sharing layer-associated morphological and functional properties, simultaneous patch clamp recordings and optogenetic studies reveal that aIP-derived neurons exhibit systematic biases in both their intralaminar monosynaptic connectivity and the post-synaptic partners that they target within deeper layers of cortex. Multiple cortical progenitor pools therefore represent an important factor in establishing diversity amongst local and long-range fine-scale glutamatergic connectivity, which generates subnetworks for routing excitatory synaptic information.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Potenciales de Acción / Neocórtex / Potenciales Postsinápticos Excitadores / Red Nerviosa / Neuronas Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Potenciales de Acción / Neocórtex / Potenciales Postsinápticos Excitadores / Red Nerviosa / Neuronas Límite: Animals Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido