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Grape Seed Polyphenols Ameliorated Dextran Sulfate Sodium-Induced Colitis via Suppression of Inflammation and Apoptosis.
Wang, Yunxia; Wang, Yunan; Shen, Wanli; Wang, Yandi; Cao, Yini; Nuerbulati, Nuerdida; Chen, Wen; Lu, Guotao; Xiao, Weiming; Qi, Rong.
Afiliación
  • Wang Y; Peking University Institute of Cardiovascular Sciences, Peking University Health Science Center, Peking University, Beijing, China.
  • Wang Y; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, China.
  • Shen W; Beijing Key Laboratory of Molecular Pharmaceutics and New Drug Delivery Systems, Beijing, China.
  • Wang Y; Peking University Institute of Cardiovascular Sciences, Peking University Health Science Center, Peking University, Beijing, China.
  • Cao Y; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, China.
  • Nuerbulati N; School of Pharmacy, Shihezi University, Shihezi, China.
  • Chen W; Peking University Institute of Cardiovascular Sciences, Peking University Health Science Center, Peking University, Beijing, China.
  • Lu G; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, China.
  • Xiao W; Peking University Institute of Cardiovascular Sciences, Peking University Health Science Center, Peking University, Beijing, China.
  • Qi R; Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing, China.
Pharmacology ; 105(1-2): 9-18, 2020.
Article en En | MEDLINE | ID: mdl-31743904
BACKGROUND: Ulcerative colitis (UC) is an inflammatory bowel disease. Its onset is typically gradual, usually followed by periods of spontaneous remission and subsequent relapses. Grape seed polyphenols (GSP), a natural product extracted from grape seeds, have strong anti-inflammatory functions. OBJECTIVES: In this study, we investigated whether GSP has an inhibitory effect on UC and its related mechanism or not. METHODS: We induced UC by 2.5% dextran sulfate sodium (DSS) and GSP at different doses (500 and 750 mg/kg body weight per day) was administrated to the mice by gavage. Body weight, diarrhea, and bloody stool were recorded every day to evaluate disease activity index. Hemotoxylin-eosin staining and immunohistochemical staining were used to identify the histological damages and inflammatory infiltration in colon tissues. Real-time polymerase chain reaction was used to evaluate mRNA expression of interleukin (IL)-6, IL-1ß, and tumor necrosis factor (TNF)-α and the expression of phosphorylated-signal transducer and activator of transcription 3 (STAT3) and STAT3 were assessed by western blot. The immunofluorescent assay was used to evaluate the apoptosis of intestinal epithelial cells (IECs). RESULTS: GSP could alleviate the loss of body weight, diarrhea, bloody stool, the mucosal damage, and inflammatory infiltration. GSP could also downregulate the mRNA expression of inflammatory cytokines IL-6, IL-1ß, and TNF-α as well as the phosphorylation of STAT3 and ameliorate the apoptosis of IECs. CONCLUSIONS: Our study suggests that GSP has protective effects against DSS-induced UC, which may through suppression of inflammation and apoptosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Vitis / Polifenoles / Antiinflamatorios Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Pharmacology Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Colitis Ulcerosa / Vitis / Polifenoles / Antiinflamatorios Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Pharmacology Año: 2020 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza