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Conserved HA-peptides expressed along with flagellin in Trichoplusia ni larvae protects chicken against intranasal H7N1 HPAIV challenge.
Sisteré-Oró, Marta; Martínez-Pulgarín, Susana; Solanes, David; Veljkovic, Veljko; López-Serrano, Sergi; Córdoba, Lorena; Cordón, Ivan; Escribano, José M; Darji, Ayub.
Afiliación
  • Sisteré-Oró M; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
  • Martínez-Pulgarín S; Alternative Gene Expression S.L. ALGENEX, Centro empresarial - Parque Científico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain.
  • Solanes D; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: david.solanes@irta.cat.
  • Veljkovic V; Biomed Protection, Galveston, 77550 TX, USA.
  • López-Serrano S; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: sergi.lopez@irta.cat.
  • Córdoba L; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: lorena.cordoba@irta.cat.
  • Cordón I; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ivan.cordon@irta.cat.
  • Escribano JM; Alternative Gene Expression S.L. ALGENEX, Centro empresarial - Parque Científico y Tecnológico Universidad Politécnica de Madrid Campus de Montegancedo, 28223 Pozuelo de Alarcón, Spain.
  • Darji A; IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain. Electronic address: ayub.darji@irta.cat.
Vaccine ; 38(3): 416-422, 2020 01 16.
Article en En | MEDLINE | ID: mdl-31735501
The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing "flagellin-NG34/CS17 antigen" were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CS17 vaccine protected the vaccinated chickens and blocked viral shedding orally and cloacally. Furthermore, no apparent clinical signs were monitored in 10/12 vaccinated individuals. The mechanism of protection conferred is under investigation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas Hemaglutininas del Virus de la Influenza / Granulovirus / Subtipo H7N1 del Virus de la Influenza A / Flagelina / Gripe Aviar Límite: Animals Idioma: En Revista: Vaccine Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas Hemaglutininas del Virus de la Influenza / Granulovirus / Subtipo H7N1 del Virus de la Influenza A / Flagelina / Gripe Aviar Límite: Animals Idioma: En Revista: Vaccine Año: 2020 Tipo del documento: Article País de afiliación: España Pais de publicación: Países Bajos