Microcalorimetric determination of the kinetics of substrate utilisation by non-growing suspensions of Neisseria sicca.
Microbios
; 54(218): 15-21, 1988.
Article
en En
| MEDLINE
| ID: mdl-3173127
The metabolism of various substrates by non-growing suspensions of Neisseria sicca was investigated by a flow-microcalorimetric technique. Substrate utilisation showed Michaelis kinetics allowing determination of saturation constants (Km) and maximum specific rates of substrate utilisation (Vmax). Pyruvate, lactate, a number of tricarboxylic acid cycle intermediates, and amino acids (aspartate, glutamate and proline) were rapidly metabolised [Vmax 5-35 mumol (g dry wt cells)-1 min-1]; Km values were between 4 and 20 microM. Glucose, glycerol, acetate and the other amino acids investigated gave only a slight or no increase in power. The pattern of substrate utilisation is discussed in relation to the role of carbonic anhydrase in N. sicca.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neisseria
Idioma:
En
Revista:
Microbios
Año:
1988
Tipo del documento:
Article
País de afiliación:
Reino Unido
Pais de publicación:
Reino Unido