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Identification of lncRNAs by RNA Sequencing Analysis During in Vivo Pre-Implantation Developmental Transformation in the Goat.
Ling, Ying-Hui; Zheng, Qi; Li, Yun-Sheng; Sui, Meng-Hua; Wu, Hao; Zhang, Yun-Hai; Chu, Ming-Xing; Ma, Yue-Hui; Fang, Fu-Gui; Xu, Li-Na.
Afiliación
  • Ling YH; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Zheng Q; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, China.
  • Li YS; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Sui MH; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, China.
  • Wu H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Zhang YH; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, China.
  • Chu MX; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Ma YH; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, China.
  • Fang FG; College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
  • Xu LN; Local Animal Genetic Resources Conservation and Biobreeding Laboratory of Anhui Province, Hefei, China.
Front Genet ; 10: 1040, 2019.
Article en En | MEDLINE | ID: mdl-31708972
Pre-implantation development is a dynamic, complex and precisely regulated process that is critical for mammalian development. There is currently no description of the role of the long noncoding RNAs (lncRNAs) during the pre-implantation stages in the goat. The in vivo transcriptomes of oocytes (n = 3) and pre-implantation stages (n=19) at seven developmental stages in the goat were analyzed by RNA sequencing (RNA-Seq). The major zygotic gene activation (ZGA) event was found to occur between the 8- and 16-cell stages in the pre-implantation stages. We identified 5,160 differentially expressed lncRNAs (DELs) in developmental stage comparisons and functional analyses of the major and minor ZGAs. Fourteen lncRNA modules were found corresponding to specific pre-implantation developmental stages by weighted gene co-expression network analysis (WGCNA). A comprehensive analysis of the lncRNAs at each developmental transition of high correlation modules was done. We also identified lncRNA-mRNA networks and hub-lncRNAs for the high correlation modules at each stage. The extensive association of lncRNA target genes with other embryonic genes suggests an important regulatory role for lncRNAs in embryonic development. These data will facilitate further exploration of the role of lncRNAs in the developmental transformation in the pre-implantation stage.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Front Genet Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Front Genet Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza