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Inhibition by Marine Algae of Chikungunya Virus Isolated From Patients in a Recent Disease Outbreak in Rio de Janeiro.
Cirne-Santos, Claudio Cesar; Barros, Caroline de Souza; Nogueira, Caio Cesar Richter; Azevedo, Renata Campos; Yamamoto, Kristie Aimi; Meira, Guilherme Louzada Silva; de Vasconcelos, Zilton Farias Meira; Ratcliffe, Norman Arthur; Teixeira, Valéria Laneuville; Schmidt-Chanasit, Jonas; Ferreira, Davis Fernandes; Paixão, Izabel Christina Nunes de Palmer.
Afiliación
  • Cirne-Santos CC; Laboratório de Virologia Molecular e Biotecnologia Marinha, Programa de Pós-graduação em Ciências e Biotecnologia, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.
  • Barros CS; Departamento de Ensino, Curso de Farmácia na Universidade Salgado de Oliveira, Niterói, Brazil.
  • Nogueira CCR; Laboratório de Virologia Molecular e Biotecnologia Marinha, Programa de Pós-graduação em Ciências e Biotecnologia, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.
  • Azevedo RC; Laboratório de Virologia Molecular e Biotecnologia Marinha, Programa de Pós-graduação em Ciências e Biotecnologia, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.
  • Yamamoto KA; Laboratório de Produtos Naturais de Algas Marinhas (ALGAMAR), Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.
  • Meira GLS; Instituto de Microbiologia Paulo de Góes (IMPPG), Departamento de Virologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
  • de Vasconcelos ZFM; Instituto de Microbiologia Paulo de Góes (IMPPG), Departamento de Virologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
  • Ratcliffe NA; Instituto de Microbiologia Paulo de Góes (IMPPG), Departamento de Virologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
  • Teixeira VL; Instituto Fernandes Figueira (IFF), Fundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, Brazil.
  • Schmidt-Chanasit J; Department of Biosciences, College of Science, Swansea University, Swansea, United Kingdom.
  • Ferreira DF; Laboratório de Produtos Naturais de Algas Marinhas (ALGAMAR), Departamento de Biologia Marinha, Instituto de Biologia, Universidade Federal Fluminense, Niterói, Brazil.
  • Paixão ICNP; Laboratório de Biologia e Taxonomia de Algas (LABIOTAL), Programa de Pós-graduação em Biodiversidade Neotropical, Instituto de Biociencias, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Front Microbiol ; 10: 2426, 2019.
Article en En | MEDLINE | ID: mdl-31708898
Chikungunya virus (CHIKV) infection is one of the most challenging re-emergent diseases caused by a virus, and with no specific antiviral treatment it has now become a major public health concern. In this investigation, 25 blood samples were collected from patients with characteristic CHIKV symptoms and submitted to a virus isolation protocol, which detected 3 CHIKV isolates. These samples were evaluated by sequencing for the characterization of the strains and any homology to viruses circulating in Brazil during a recent outbreak. These viruses were used for the development of antiviral assays. Subsequently, the inhibitory effects of seaweed extracts on CHIKV replication were studied. The marine species of algae tested were Bryothamnion triquetrum, Caulerpa racemosa, Laurencia dendroidea, Osmundaria obtusiloba, Ulva fasciata, and Kappaphycus alvarezii, all of which are found in different countries including Brazil. The results revealed high levels of CHIKV inhibition, including extracts of O. obtusiloba with inhibition values of 1.25 µg/mL and a selectivity index of 420. Viral inhibition was dependent on the time of addition of extract of O. obtusiloba to the infected cells, with the optimal inhibition occurring up to 16 h after infection. Neuron evaluations with O. obtusiloba were performed and demonstrated low toxicity, and in infected neurons we observed high inhibitory activity in a dose-dependent manner. These results indicate that the algal extracts may be promising novel candidates for the development of therapeutic agents against CHIKV infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Front Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Front Microbiol Año: 2019 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza