A colorimetric detection of microRNA-148a in gastric cancer by gold nanoparticle-RNA conjugates.
Nanotechnology
; 31(9): 095501, 2020 Feb 21.
Article
en En
| MEDLINE
| ID: mdl-31703221
For the early diagnosis of gastric cancer, microRNA-148a (miRNA-148a) as a promising biomarker is measured by a simple colorimetric biosensor due to its unique surface plasmon resonance (SPR) absorption of gold nanoparticles (AuNPs). In the assay system, the sensing probes are facilitated by the conjugation of AuNPs with RNA probes (RNAP) via Au-S bonds, which align in a tail-to-tail fashion onto the target RNA. When miRNA-148a is introduced, a sandwich hybridization reaction is triggered between the AuNP-RNAP conjugates and targets, resulting in changes in the SPR absorption band, microscopic distribution and macroscopic color of the AuNP solution. Following this principle, this colorimetric method is able to quantitatively detect miRNA-148a at nanomolar level with a limit of â¼1.9 nM, and exhibits high sensitivity and selectivity by a low-cost UV-vis spectrometer or even the naked eye. Moreover, the AuNP network materials with a characteristic sharp 'melting transition' provide significant guidance for the reusability of DNA or RNA biosensors.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
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Técnicas Biosensibles
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Sondas ARN
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MicroARNs
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Nanopartículas del Metal
Tipo de estudio:
Diagnostic_studies
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Guideline
/
Screening_studies
Límite:
Humans
Idioma:
En
Revista:
Nanotechnology
Año:
2020
Tipo del documento:
Article
Pais de publicación:
Reino Unido