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Indomethacin enhances anti-tumor efficacy of a MUC1 peptide vaccine against breast cancer in MUC1 transgenic mice.
Curry, Jennifer M; Besmer, Dahlia M; Erick, Timothy K; Steuerwald, Nury; Das Roy, Lopamudra; Grover, Priyanka; Rao, Shanti; Nath, Sritama; Ferrier, Jacob W; Reid, Robert W; Mukherjee, Pinku.
Afiliación
  • Curry JM; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Besmer DM; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Erick TK; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Steuerwald N; Molecular Biology and Genomics Laboratory, Carolinas Medical Center, Charlotte, NC, United States of America.
  • Das Roy L; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Grover P; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Rao S; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Nath S; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Ferrier JW; Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Reid RW; Department of Bioinformatics and Genomics, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
  • Mukherjee P; Department of Biological Sciences, University of North Carolina at Charlotte, Charlotte, NC, United States of America.
PLoS One ; 14(11): e0224309, 2019.
Article en En | MEDLINE | ID: mdl-31693710
In recent years, vaccines against tumor antigens have shown potential for combating invasive cancers, including primary tumors and metastatic lesions. This is particularly pertinent for breast cancer, which is the second-leading cause of cancer-related death in women. MUC1 is a glycoprotein that is normally expressed on glandular epithelium, but is overexpressed and under-glycosylated in most human cancers, including the majority of breast cancers. This under-glycosylation exposes the MUC1 protein core on the tumor-associated form of the protein. We have previously shown that a vaccine consisting of MUC1 core peptides stimulates a tumor-specific immune response. However, this immune response is dampened by the immunosuppressive microenvironment within breast tumors. Thus, in the present study, we investigated the effectiveness of MUC1 vaccination in combination with four different drugs that inhibit different components of the COX pathway: indomethacin (COX-1 and COX-2 inhibitor), celecoxib (COX-2 inhibitor), 1-methyl tryptophan (indoleamine 2,3 dioxygenase inhibitor), and AH6809 (prostaglandin E2 receptor antagonist). These treatment regimens were explored for the treatment of orthotopic MUC1-expressing breast tumors in mice transgenic for human MUC1. We found that the combination of vaccine and indomethacin resulted in a significant reduction in tumor burden. Indomethacin did not increase tumor-specific immune responses over vaccine alone, but rather appeared to reduce the proliferation and increase apoptosis of tumor cells, thus rendering them susceptible to immune cell killing.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Indometacina / Inhibidores de la Ciclooxigenasa / Mucina-1 / Vacunas contra el Cáncer / Neoplasias Mamarias Experimentales Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Indometacina / Inhibidores de la Ciclooxigenasa / Mucina-1 / Vacunas contra el Cáncer / Neoplasias Mamarias Experimentales Límite: Animals / Female / Humans Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos