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Development and evaluation of a colloidal gold immunochromatographic assay based on recombinant protein CatL1D for serodiagnosis of sheep fasciolosis.
Xifeng, W; Mengfan, Q; Kai, Z; Guowu, Z; Jing, L; Lixia, W; Jun, Q; Qingling, M; Shasha, G; Yunfu, H; Xuepeng, C.
Afiliación
  • Xifeng W; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Mengfan Q; School of Biotechnology, Central South University, Changsha, Hunan 410012, China.
  • Kai Z; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Guowu Z; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Jing L; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Lixia W; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Jun Q; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Qingling M; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Shasha G; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Yunfu H; College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.
  • Xuepeng C; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China.
J Helminthol ; 94: e98, 2019 Nov 04.
Article en En | MEDLINE | ID: mdl-31679525
Fasciolosis is a zoonotic parasitic disease that seriously endangers the development of animal husbandry and human health. In order to develop a rapid serological diagnostic method for fasciolosis in ruminants, the CatL1D and CatB4 genes of Fasciola hepatica were amplified by reverse transcription polymerase chain reaction (PCR) and cloned, respectively, and then the CatL-B fusion gene (MeCatL-B) was constructed by gene splicing by overlap extension PCR technique. The recombinant rCatL1D, rCatB4 and rMeCatL-B proteins were then prepared by prokaryotic expression, respectively, and the recombinant protein with high specificity and sensitivity was screened via indirect enzyme-linked immunosorbent assay. Using the selected recombinant protein rCatL1D as a diagnostic antigen, we developed a colloidal gold immunochromatographic assay (CGIA) for detecting F. hepatica-specific antibodies, and 426 serum samples of slaughtered sheep were used to evaluate the sensitivity and specificity of F. hepatica CGIA assay. The results showed that the sensitivity and specificity of rCatL1D protein (100%, 96.67%) were higher than those of rCatB4 (94.29%, 80%) and rMeCatL-B (91.43%, 90%). Compared with the gold standard post-mortem inspection, the specificity and sensitivity of the CGIA method was 100% and 97%, respectively, and the consistency rate between these two methods was 99.3%. These results confirmed that the CGIA method based on rCatL1D protein could be a promising approach for rapid diagnosis of sheep fasciolosis because of its high sensitivity and specificity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Inmunoensayo / Proteínas del Helminto / Fasciola hepatica / Fascioliasis Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: J Helminthol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Ovejas / Inmunoensayo / Proteínas del Helminto / Fasciola hepatica / Fascioliasis Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Animals Idioma: En Revista: J Helminthol Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido