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Effect of cell disruption methods on the extraction of bioactive metabolites from microalgal biomass.
Stirk, Wendy A; Bálint, Péter; Vambe, McMaster; Lovász, Csaba; Molnár, Zoltán; van Staden, Johannes; Ördög, Vince.
Afiliación
  • Stirk WA; Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, P/Bag X 01, Scottsville, 3209, South Africa. Electronic address: stirk@ukzn.ac.za.
  • Bálint P; Department of Plant Sciences, Faculty of Agricultural and Food Sciences, Széchenyi István University, Kolbai K. Str. 8, H-9200, Mosonmagyaróvár, Hungary. Electronic address: balint.peter@sze.hu.
  • Vambe M; Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, P/Bag X 01, Scottsville, 3209, South Africa. Electronic address: mcmastervambe@gmail.com.
  • Lovász C; WESSLING Hungary Ltd., Anonymus Str. 6, H-1045, Budapest, Hungary. Electronic address: lovasz.csaba@wessling.hu.
  • Molnár Z; Department of Plant Sciences, Faculty of Agricultural and Food Sciences, Széchenyi István University, Kolbai K. Str. 8, H-9200, Mosonmagyaróvár, Hungary. Electronic address: molnar.zoltan@sze.hu.
  • van Staden J; Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, P/Bag X 01, Scottsville, 3209, South Africa. Electronic address: rcpgd@ukzn.ac.za.
  • Ördög V; Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal, Pietermaritzburg, P/Bag X 01, Scottsville, 3209, South Africa; Department of Plant Sciences, Faculty of Agricultural and Food Sciences, Széchenyi István University, Kolbai K. Str. 8, H-9200, Moson
J Biotechnol ; 307: 35-43, 2020 Jan 10.
Article en En | MEDLINE | ID: mdl-31678206
Microalgae synthesize a variety of potentially high-value compounds. Due to their robust cell wall, cell disruption is necessary to improve extraction of these compounds. While cell disruption methods have been optimized for lipid and protein extraction, there are limited studies for other bioactive compounds. The present study investigated the effect of freeze-drying combined with sonication or ball-milling on the extraction of antioxidant and plant biostimulating compounds from Chlorella sp., Chlorella vulgaris and Scenedesmus acutus. Both cell disruption methods resulted in higher extract yields from the biomass compared to freeze-dried biomass using 50% methanol as a solvent. Antioxidant activity of Chlorella extracts was generally higher than freeze-dried extracts based on the diphenylpicrylhydrazyl (DPPH) and ß-carotene linoleic acid assays. However, the effectiveness of each treatment varied between microalgae strains. Sonication resulted in the highest antioxidant activity in Chlorella sp. extracts. Ball-milling gave the best results for C. vulgaris extracts in the DPPH assay. Both cell disruption methods decreased antioxidant activity in S. acutus extracts. Plant biostimulating activity was tested using the mung bean rooting assay. Damaging the membrane by freeze-drying was sufficient to release the active compounds using water extracts. In contrast, both cell disruption methods negatively affected the biological activity of the extracts. These results indicate that bioactive compounds in microalgae are sensitive to post-harvest processes and their biological activity can be negatively affected by cell disruption methods. Care must be taken to not only optimize yield but to also preserve the biological activity of the target compounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sonicación / Extractos Vegetales / Scenedesmus / Chlorella vulgaris / Microalgas / Fitoquímicos / Antioxidantes Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sonicación / Extractos Vegetales / Scenedesmus / Chlorella vulgaris / Microalgas / Fitoquímicos / Antioxidantes Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2020 Tipo del documento: Article Pais de publicación: Países Bajos