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n-Butylparaben exposure through gestation and lactation impairs spermatogenesis and steroidogenesis causing reduced fertility in the F1 generation male rats.
Maske, Priyanka; Dighe, Vikas; Mote, Chandrashekhar; Vanage, Geeta.
Afiliación
  • Maske P; National Centre for Preclinical Reproductive and Genetic Toxicology, ICMR-National Institute for Research in Reproductive Health, Parel, Mumbai, Maharashtra, India.
  • Dighe V; National Centre for Preclinical Reproductive and Genetic Toxicology, ICMR-National Institute for Research in Reproductive Health, Parel, Mumbai, Maharashtra, India.
  • Mote C; Krantisinh Nana Patil College of Veterinary Science, Shirval, Maharashtra, India.
  • Vanage G; National Centre for Preclinical Reproductive and Genetic Toxicology, ICMR-National Institute for Research in Reproductive Health, Parel, Mumbai, Maharashtra, India. Electronic address: geetavanage@gmail.com.
Environ Pollut ; 256: 112957, 2020 Jan.
Article en En | MEDLINE | ID: mdl-31672375
Parabens are class of preservatives used in vast majority of commercial products, and a potential Endocrine Disrupting Chemical (EDC). The present study was undertaken to delineate the effects of n-butylparaben on F1 male progeny exposed maternally through gestation and lactation via subcutaneous route. The F0 dams were given subcutaneous injections of n-butylparaben from gestation day (GD) 6 to postnatal day (PND) 21 with doses of 10, 100, 1000 mg/kg Bw/day in corn oil. The F1 male rats were monitored for pubertal development and sexual maturation; these were sacrificed on PND 30, 45 and 75. On PND 75, these F1 male rats were subjected for fertility assessment with unexposed female rats. A delayed testicular descent at 100 and 1000 mg/kg Bw dose and delayed preputial separation at 10 mg/kg Bw dose was observed in exposed F1 male rats. Decreased sperm count, motility and Daily Sperm Production was observed at 100 mg/kg Bw dose at PND 75. Interestingly, the sperm transit time in the epididymis was accelerated at this dose. Significant perturbed testicular expression of steroid receptors (ERα and ß, AR), INSL3 and StAR genes with increased T and LH levels indicates direct effect on spermatogenesis and steroidogenesis. These F1 generation adult rats were sub-fertile with increased (%) pre- and post-implantation loss at 100 and 1000 mg/kg Bw/day dose. This is the first report on n-butylparaben highlighting the involvement of testicular leydig cells with accelerated sperm transit time leading to reduced fertility in the maternally exposed F1 male rats through estrogenic/anti-androgenic action.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parabenos / Efectos Tardíos de la Exposición Prenatal / Espermatogénesis / Receptores de Esteroides / Disruptores Endocrinos / Fertilidad Límite: Animals / Pregnancy Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Parabenos / Efectos Tardíos de la Exposición Prenatal / Espermatogénesis / Receptores de Esteroides / Disruptores Endocrinos / Fertilidad Límite: Animals / Pregnancy Idioma: En Revista: Environ Pollut Asunto de la revista: SAUDE AMBIENTAL Año: 2020 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido