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Protective Effect of PBCA Nanoparticles Loaded with Thymulin Against the Relapsing-Remitting Form of Experimental Autoimmune Encephalomyelitis in Mice.
Lunin, Sergey M; Khrenov, Maxim O; Glushkova, Olga V; Parfenyuk, Svetlana B; Novoselova, Tatyana V; Novoselova, Elena G.
Afiliación
  • Lunin SM; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. lunin@rambler.ru.
  • Khrenov MO; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. xpehob2004@mail.ru.
  • Glushkova OV; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. glushckova@mail.ru.
  • Parfenyuk SB; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. lana_kras2@rambler.ru.
  • Novoselova TV; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. novossulova_t@rambler.ru.
  • Novoselova EG; Institute of Cell Biophysics of the Russian Academy of Sciences, PSCBR RAS, Institutskaya str. 3, Pushchino, 142290 Moscow, Russia. elenanov_06@mail.ru.
Int J Mol Sci ; 20(21)2019 Oct 29.
Article en En | MEDLINE | ID: mdl-31671728
Relapsing-remitting experimental autoimmune encephalomyelitis (rEAE) in mice is a model that closely resembles relapsing-remitting multiple sclerosis in humans. This study aims to investigate a new approach to modulation of the inflammatory response in rEAE mice using a thymic peptide thymulin bound to polybutylcyanoacrylate (PBCA) nanoparticles. PBCA nanoparticles were used to prolong the presence of thymulin in the blood. Cytokine levels in blood were measured by ELISA; NF-κB and SAPK/JNK cascade activation, as well as Hsp72 and p53 protein expression, were measured by Western blotting. Animal health statuses were estimated using severity scores. Results showed that the cytokine response in rEAE was multi-staged: an early phase was accompanied by an increase in plasma interferon-γ, while the interleukin (IL)-17 response was markedly increased at a later stage. The stages were attributed to rEAE induction and maintenance phases. Thymulin significantly alleviated symptoms of rEAE and lowered plasma cytokine levels both in early and later stages of rEAE, and decreased NF-κB and SAPK/JNK cascade activation. Thymulin modulated NF-kappaB pathway activity via site-specific phosphorylation of RelA/p65 protein (at Ser276 and Ser536). The effect of nanoparticle-bound thymulin was more pronounced than the effect of free thymulin. Therefore, PBCA-thymulin can be considered a prospective treatment for this pathology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Tímico Circulante / Esclerosis Múltiple Recurrente-Remitente / Enbucrilato / Encefalomielitis Autoinmune Experimental / Nanopartículas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factor Tímico Circulante / Esclerosis Múltiple Recurrente-Remitente / Enbucrilato / Encefalomielitis Autoinmune Experimental / Nanopartículas Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2019 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza