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Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo.
Dahlin, Lukas R; Gerritsen, Alida T; Henard, Calvin A; Van Wychen, Stefanie; Linger, Jeffrey G; Kunde, Yuliya; Hovde, Blake T; Starkenburg, Shawn R; Posewitz, Matthew C; Guarnieri, Michael T.
Afiliación
  • Dahlin LR; 1Department of Chemistry, Colorado School of Mines, Golden, CO 80401 USA.
  • Gerritsen AT; 2Computational Science Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
  • Henard CA; 3National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
  • Van Wychen S; 3National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
  • Linger JG; 3National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
  • Kunde Y; 4Los Alamos National Laboratory, Los Alamos, NM 87545 USA.
  • Hovde BT; 4Los Alamos National Laboratory, Los Alamos, NM 87545 USA.
  • Starkenburg SR; 4Los Alamos National Laboratory, Los Alamos, NM 87545 USA.
  • Posewitz MC; 1Department of Chemistry, Colorado School of Mines, Golden, CO 80401 USA.
  • Guarnieri MT; 3National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401 USA.
Commun Biol ; 2: 388, 2019.
Article en En | MEDLINE | ID: mdl-31667362
Microalgae are promising biocatalysts for applications in sustainable fuel, food, and chemical production. Here, we describe culture collection screening, down-selection, and development of a high-productivity, halophilic, thermotolerant microalga, Picochlorum renovo. This microalga displays a rapid growth rate and high diel biomass productivity (34 g m-2 day-1), with a composition well-suited for downstream processing. P. renovo exhibits broad salinity tolerance (growth at 107.5 g L-1 salinity) and thermotolerance (growth up to 40 °C), beneficial traits for outdoor cultivation. We report complete genome sequencing and analysis, and genetic tool development suitable for expression of transgenes inserted into the nuclear or chloroplast genomes. We further evaluate mechanisms of halotolerance via comparative transcriptomics, identifying novel genes differentially regulated in response to high salinity cultivation. These findings will enable basic science inquiries into control mechanisms governing Picochlorum biology and lay the foundation for development of a microalga with industrially relevant traits as a model photobiology platform.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlorophyta / Microalgas Tipo de estudio: Prognostic_studies Idioma: En Revista: Commun Biol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Chlorophyta / Microalgas Tipo de estudio: Prognostic_studies Idioma: En Revista: Commun Biol Año: 2019 Tipo del documento: Article Pais de publicación: Reino Unido