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Antityrosinase mechanism of ellagic acid in vitro and its effect on mouse melanoma cells.
Huang, Qian; Chai, Wei-Ming; Ma, Zuo-Yuan; Deng, Wei-Liang; Wei, Qi-Ming; Song, Shuang; Zou, Zheng-Rong; Peng, Yi-Yuan.
Afiliación
  • Huang Q; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Chai WM; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Ma ZY; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Deng WL; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Wei QM; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Song S; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Zou ZR; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
  • Peng YY; College of Life Science, and Key Laboratory of Functional Small Organic Molecule, Ministry of Education, Jiangxi Normal University, Nanchang, China.
J Food Biochem ; 43(11): e12996, 2019 11.
Article en En | MEDLINE | ID: mdl-31659813
The activities of ellagic acid in inhibiting mushroom tyrosinase and cell proliferation were evaluated in this research. The results of enzyme kinetics indicated that ellagic acid could effectively inhibit tyrosinase activity. The value of the semi-inhibitory rate (IC50 ) was 0.2 ± 0.05 mM. Ellagic acid inhibited tyrosinase activity in a reversible manner and was a mixed tyrosinase inhibitor. Furthermore, ellagic acid had a good inhibitory effect on the proliferation of mouse melanoma B16 cells and could induce apoptosis. The results acquired from fluorescence spectroscopy revealed that the interaction of ellagic acid with tyrosinase depended on hydrogen bond and electrostatic force. In addition, computational docking showed that ellagic acid interacted with amino acid residues of tyrosinase (Asn19 and Lys372) by hydrogen bond and produced electrostatic interaction with amino residue Lys18. PRACTICAL APPLICATIONS: In the present research, the antityrosinase mechanism of ellagic acid and its effect on mouse melanoma cells were investigated. This study suggested that ellagic acid had a strong inhibitory activity against tyrosinase and cell proliferation,which laid an experimental foundation for the development of new drugs and whitening products. The combined multispectral methods used in this research can be applied to the screening of other antityrosinase inhibitors, further promoting the development and utilization of tyrosinase inhibitors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agaricales / Ácido Elágico / Melanoma Límite: Animals Idioma: En Revista: J Food Biochem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agaricales / Ácido Elágico / Melanoma Límite: Animals Idioma: En Revista: J Food Biochem Año: 2019 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos