Tomato seeds pretreated with Antifreeze protein type I (AFP I) promotes the germination under cold stress by regulating the genes involved in germination process.

Kyu, Swum Yi; Naing, Aung Htay; Pe, Phyo Phyo Win; Park, Kyeung Il; Kim, Chang Kil

Kyu, Swum Yi; Naing, Aung Htay; Pe, Phyo Phyo Win; Park, Kyeung Il; Kim, Chang Kil.

Afiliación

Kyu SY; Department of Horticultural Science, Kyungpook National University, Daegu, South Korea.
Naing AH; Department of Horticultural Science, Kyungpook National University, Daegu, South Korea.
Pe PPW; Department of Horticulture and Life science, Yeungnam University, Gyeongsan, South Korea.
Park KI; Department of Horticulture and Life science, Yeungnam University, Gyeongsan, South Korea.
Kim CK; Department of Horticultural Science, Kyungpook National University, Daegu, South Korea.

Plant Signal Behav ; 14(12): 1682796, 2019.

Article en En | MEDLINE | ID: mdl-31647356

RESUMEN

This study was conducted to investigate the involvement of antifreeze proteins (AFPs; type I and III) in the germination mechanism of tomato seeds under low temperature stress. Germination of the seeds grown at a room temperature (25°C) was observed on 5 days after sowing (DAS), while all seeds exposed to a low temperature started to germinate at 16 days after sowing (DAS). However, in comparison with control seeds (0 µg/l), seeds treated with AFP I (100, 300, or 500 µg/l) germinated earlier and at a higher percentage until 20 DAS, and seeds treated with 100 µg/l AFP I showed the highest percentage of germination. Surprisingly, AFP III did not significantly increase germination, and the rate was lower among 500 µg/l AFP III-treated seeds compared with control seeds (0 µg/l). The transcription levels of the plasma membrane-associated H+-ATPase gene and antioxidant-related superoxide dismutase (SOD) and catalase 1 (CAT1) genes were analyzed, and the transcription levels of the genes in the seeds grown at 25°C were relatively low. For low temperature-treated seeds, H+-ATPase in control seeds (0 µg/l) was higher compared with that in AFP I-treated seeds and was lower compared with that in AFP III-treated seeds. The expression levels of the antioxidant-related genes (SOD and CAT1) were lower in AFP I-treated seeds than in control seeds (0 µg/l); however, they were higher in AFP III-treated seeds than in control seeds (0 µg/l). Overall, compared with AFP III, AFP I may potentially function as a cold-protective agent by modulating the genes associated with seed germination.

Asunto(s)

Proteínas Anticongelantes/farmacología; Respuesta al Choque por Frío/genética; Regulación de la Expresión Génica de las Plantas/efectos de los fármacos; Germinación/genética; Semillas/genética; Solanum lycopersicum/embriología; Solanum lycopersicum/genética; Animales; Proteínas Anticongelantes/química; Respuesta al Choque por Frío/efectos de los fármacos; Peces; Germinación/efectos de los fármacos; Solanum lycopersicum/efectos de los fármacos; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; ATPasas de Translocación de Protón/metabolismo; ARN Mensajero/genética; ARN Mensajero/metabolismo; Plantones/anatomía & histología; Plantones/efectos de los fármacos; Plantones/genética; Semillas/embriología; Transcripción Genética/efectos de los fármacos

Palabras clave

Antifreeze protein; gene expression; germination; low temperature; tomato

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semillas / Solanum lycopersicum / Germinación / Regulación de la Expresión Génica de las Plantas / Proteínas Anticongelantes / Respuesta al Choque por Frío Límite: Animals Idioma: En Revista: Plant Signal Behav Asunto de la revista: BOTANICA / FISIOLOGIA Año: 2019 Tipo del documento: Article País de afiliación: Corea del Sur Pais de publicación: Estados Unidos

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