Enzymatic synthesis and modification of high molecular weight DNA using terminal deoxynucleotidyl transferase.

Deshpande, Sonal; Yang, Yunqi; Chilkoti, Ashutosh; Zauscher, Stefan

Deshpande, Sonal; Yang, Yunqi; Chilkoti, Ashutosh; Zauscher, Stefan.

Afiliación

Deshpande S; Department of Biomedical Engineering, Duke University, Durham, NC, United States.
Yang Y; Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, United States.
Chilkoti A; Department of Biomedical Engineering, Duke University, Durham, NC, United States; Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, United States. Electronic address: chilkoti@duke.edu.
Zauscher S; Department of Biomedical Engineering, Duke University, Durham, NC, United States; Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC, United States. Electronic address: zauscher@duke.edu.

Methods Enzymol ; 627: 163-188, 2019.

Article en En | MEDLINE | ID: mdl-31630739

Asunto(s)

ADN Nucleotidilexotransferasa/metabolismo; ADN/biosíntesis; ADN/metabolismo; Peso Molecular; Polimerizacion

Palabras clave

Deoxynucleotidyl triphosphates (dNTPs); Deoxyribonucleic acid (DNA); Surface initiated enzymatic polymerization (SIEP); TdT-catalyzed enzymatic polymerization (TcEP); Terminal deoxynucleotidyl transferase (TdT); Unnatural nucleotides

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN Nucleotidilexotransferasa Tipo de estudio: Guideline Idioma: En Revista: Methods Enzymol Año: 2019 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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