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Water Extracts of Hull-less Waxy Barley (Hordeum vulgare L.) Cultivar 'Boseokchal' Inhibit RANKL-induced Osteoclastogenesis.
Kim, Kwang-Jin; Lee, Yongjin; Son, So-Ri; Lee, Hyunjin; Son, Young-Jin; Lee, Mi-Kyung; Lee, Mija.
Afiliación
  • Kim KJ; Department of Pharmacy, Sunchon National University, Suncheon 57922, Korea. mastiffk@naver.com.
  • Lee Y; Department of Pharmacy, Sunchon National University, Suncheon 57922, Korea. yojilee@gmail.com.
  • Son SR; Department of Biomedical Science and technology, Graduate School, Kyung Hee University, Seoul 02447, Korea. allosori@khu.ac.kr.
  • Lee H; Department of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Korea. poi7346@korea.kr.
  • Son YJ; Department of Pharmacy, Sunchon National University, Suncheon 57922, Korea. sony@sunchon.ac.kr.
  • Lee MK; Department of Food and Nutrition, Sunchon National University, Jeonnam, Suncheon 57922, Korea. leemk@scnu.ac.kr.
  • Lee M; Department of Crop Foundation, National Institute of Crop Science (NICS), Rural Development Administration (RDA), Wanju 55365, Korea. esilvia@korea.kr.
Molecules ; 24(20)2019 Oct 16.
Article en En | MEDLINE | ID: mdl-31623242
Osteoporosis is a disease that leads to reduced bone mineral density. The increase in patient and medical costs because of global aging is recognized as a problem. Decreased bone mass is a common symptom of bone diseases such as Paget's disease, rheumatoid arthritis, and multiple myeloma. Osteoclasts, which directly affect bone mass, show a marked increase in differentiation and activation in the aforementioned diseases. Moreover, these multinucleated cells made from monocytes/macrophages under the influence of RANKL and M-CSF, are the only cells capable of resorbing bones. In this study, we found that the water extracts of Boseokchal (BSC-W) inhibited osteoclast differentiation in vitro and investigated its inhibitory mechanism. BSC-W was obtained by extracting flour of Boseokchal using hexane and water. To osteoclast differentiation, bone marrow-derived macrophage cells (BMMs) were cultured with the vehicle (0.1% DMSO) or BSC-W in the presence of M-CSF and RANKL for 4 days. Cytotoxicity was measured by CCK-8. Gene expression of cells was confirmed by real-time PCR. Protein expression of cells was observed by western blot assay. Bone resorption activity of osteoclast evaluated by bone pit formation assay using an Osteo Assay Plate. BSC-W inhibited RANKL-induced osteoclastogenesis in a dose-dependent manner without exerting a cytotoxic effect on BMMs. BSC-W decreased the transcriptional and translational expression of c-Fos and NFATc1, which are regulators of osteoclastogenesis and reduced the mRNA expression level of TRAP, DC-STAMP, and cathepsin K, which are osteoclast differentiation marker. Furthermore, BSC-W reduced the resorption activity of osteoclasts. Taken together, our results indicate that BSC-W is a useful candidate for health functional foods or therapeutic agents that can help treat bone diseases such as osteoporosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Hordeum / Ceras / Extractos Vegetales / Ligando RANK Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Suiza
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Hordeum / Ceras / Extractos Vegetales / Ligando RANK Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2019 Tipo del documento: Article Pais de publicación: Suiza