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Antibacterial photodynamic therapy mediated by 5-aminolevulinic acid on methicillin-resistant Staphylococcus aureus.
Huang, Jianhua; Guo, Mingquan; Jin, Shengkai; Wu, Minfeng; Yang, Chen; Zhang, Guolong; Wang, Peiru; Ji, Jie; Zeng, Qingyu; Wang, Xiuli; Wang, Hongwei.
Afiliación
  • Huang J; Department of Dermatology, Huadong Hospital, Fudan University, Shanghai 200040, PR China. Electronic address: 281284528@qq.com.
  • Guo M; Shanghai Institute of Bacteriophage and Drug Resistance, Shanghai Public Health Clinical Center, Fudan University, Shanghai 201514, PR China. Electronic address: gmqjiandan@163.com.
  • Jin S; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: jinsk1223@163.com.
  • Wu M; Department of Dermatology, Huadong Hospital, Fudan University, Shanghai 200040, PR China. Electronic address: 996392167@qq.com.
  • Yang C; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: yangchen1259@163.com.
  • Zhang G; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: zglamu@163.com.
  • Wang P; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: wpeiru@qq.com.
  • Ji J; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: jier_2006@163.com.
  • Zeng Q; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: xiaojinyuhen@126.com.
  • Wang X; Shanghai Skin Disease Hospital, Institute of Photomedicine, Tongji University School of Medicine, Shanghai, PR China. Electronic address: xlwang2001@aliyun.com.
  • Wang H; Department of Dermatology, Huadong Hospital, Fudan University, Shanghai 200040, PR China. Electronic address: hongweiwang2005@aliyun.com.
Photodiagnosis Photodyn Ther ; 28: 330-337, 2019 Dec.
Article en En | MEDLINE | ID: mdl-31618677
The emergence of drug-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA), has brought great difficulties to clinical treatment. Antibacterial photodynamic therapy (aPDT) is a new non-antibiotic treatment strategy for a variety of drug-resistant bacteria. However, there are few studies on the antimicrobial mechanism of a PDT mediated by 5-aminolevulinic acid (ALA-PDT) for MRSA. In this study, we observed the bactericidal effect of ALA-PDT on MRSA. We found that ALA-PDT had the strongest bactericidal effect when ALA was at 0.05 mM, and the bactericidal activity of aPDT increased with the increase of light dose. MRSA was almost completely eliminated at 0.05 mM and 384 Jcm-2. In addition, the bactericidal morphology was also observed under a fluorescence microscope using a LIVE/DEAD® BacLight™ Bacterial Viability Kit and an electron microscope. It was also found that proteins and DNA were damaged by ALA-PDT. Finally, the transcription level of the specific gene of MRSA, nuc, was decreased by 0.74-fold (P < 0.05) after ALA-PDT treatment by qRT-PCR analysis. The findings suggest that ALA-PDT can effectively inhibit MRSA by damaging cell membrane, cytoplasm, proteins and nucleic acid.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Fármacos Fotosensibilizantes / Staphylococcus aureus Resistente a Meticilina / Ácido Aminolevulínico Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Fármacos Fotosensibilizantes / Staphylococcus aureus Resistente a Meticilina / Ácido Aminolevulínico Idioma: En Revista: Photodiagnosis Photodyn Ther Asunto de la revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Año: 2019 Tipo del documento: Article Pais de publicación: Países Bajos